Avaliação dos efeitos alelopáticos e mutagênicos de formas extrativas de Passiflora edulis Sims por meio do bioensaio Allium cepa
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Data
2009-12-15
Autores
Costalonga, Schirley Aparecida
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Universidade Federal do Espírito Santo
Resumo
The human being use plants for different things, like powerful medicine instrument and biological control. The Allium cepa system is a efficient tool for identify dangerous substances for animals and vegetables. Passiflora edulis is a native plant used abundantly as phitoteraphic in Brazil; besides it produce many substances with allelophatic potencial, but without intense studies. Thus, the aim was evaluated the allelophatic and mutagenic effects of four leave extracts and two infusions of P. edulis over A. cepa seeds treaty with continuous and discontinuous acute and chronics treatments in middle with water, three concentration of extracts (1, 2 e 5 mg/mL) or three concentrations of infusions (10, 50 e 100%). Happened a accentuated allelophatic potential for the ethanolic extract, which reduced the germination and speed’s germination index; for the hexane and acetate extracts, same results were obtained for 2 e 5 mg/mL concentration and for dichlorometane extract, only in the major concentration. Didn’t have changes on this parameters when mesocárpico and leave infusions were used. For the roots developments, none change was reported. The mitotic index was affected in all three extracts’ concentrations, in 100% of continuous treatments, all concentration of acute discontinuous and 10 e 100% of chronic treatment with mesocarpic infusion. Aneugenic effects were related only in 1 mg/mL of dichlorometane extract and 100% acute discontinuous treatment of leave infusion. Clastogenic effects happened in 2 mg/mL of acetate extract in acute discontinuous treatment and 2 mg/mL of ethanolic extract in continuous treatment. The seeds treated with 1 e 2 mg/mL of acetate extract for 72h presented abnormal roots growth and didn’t response for coloration. Happened elevated number of dead cells and in all chronic discontinuous treatment. The results above denote the deep relation between the allelophatic and mutagenic effects and reinforce the importance of study the native species. For more security concerning the use of P. edulis for human beings, advice the establish tests in animals
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Allelophaty , Mutagenicity , A. cepa system , Teste de A. cepa , P. edulis
Citação
COSTALONGA, Schirley Aparecida. Avaliação dos efeitos alelopáticos e mutagênicos de formas extrativas de Passiflora edulis Sims por meio do bioensaio Allium cepa. 2009. 85 f. Dissertação (Mestrado em Biologia Vegetal) - Universidade Federal do Espírito Santo, Centro de Ciências Humanas e Naturais, Vitória, 2009.