Morfometria, germinação in vitro e ex vitro e adequação metodológica do teste de tetrazólio em sementes de Passiflora foetida var. glazziovii killip (Passifloraceae)
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Data
2013-02-25
Autores
Costa, Poliana Rangel
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Universidade Federal do Espírito Santo
Resumo
The species Passiflora foetida L. has wide medical utility and ornamental, but
information concerning their infraspecific taxon and the physiological potential of its
seeds are still scarce. Tetrazolium tests and germination are important components
for the evaluation of the physiological quality of seeds, enabling the evaluation force
and deterioration. Through the application of these methodologies is possible to
achieve the maximum levels of germination with getting quality seedlings. In this way,
aimed to characterize P. foetida var. glaziovii Killip, evaluate their behavior in vitro
and ex vitro germinal and tailor the tetrazolium test methodology. The botanical
identification and characterization were performed from the observation of relevant
characters as identification key. The ex vitro germination was held in roller and paper
Germitest at temperatures of 25 and 20-30° C. Totally devoid of seed integument
later were used in the in vitro germination tests and tetrazolium. For the in vitro
germination medium was MS being the seeds put up the different qualities of light
(white, red, red extreme and absence) and two temperatures (25 and 20-30° C).
Tetrazolium test the seeds were submerged, tetrazolium solution at a concentration
of 2.5; 5.0; 7.5 and 10 g L-1
at temperatures of 30, 35, 40 and 45° C for two hours in
the absence of light. P. foetida var. glaziovii Killip is a wild species, characterized as
herbaceous vine with glandular trichomes on its leaves, bracts and estipulas. This
plant has small seeds of approximately 4.87 mm long and 2.15 mm wide, nonphotoblástic, and germination in vitro should be performed in alternating temperature
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of 20-30° C. In the evaluation of force through the tetrazolium test the combination of
concentration of 10 g L-1
and temperature of 30° C is recommended for this species.
Descrição
Palavras-chave
Passion fruit , Wild , Silvestre , Propagation , Propagação , Force , Vigor , Tissue culture , Cultura de tecidos
Citação
COSTA, Poliana Rangel. Morfometria, germinação in vitro e ex vitro e adequação metodológica do teste de tetrazólio em sementes de Passiflora foetida var. glazziovii killip (Passifloraceae). 2013. 40 f. Dissertação (Mestrado em Agricultura Tropical) - Programa de Pós-Graduação em Agricultura Tropical, Universidade Federal do Espírito Santo, São Mateus, 2013.