Solução de dexametasona diluída em ringer com lactato versus vitamina E diluída em azeite de oliva na prevenção de aderências abdominais em ovelhas doadoras de embriões
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Data
2010-11-12
Autores
Santos, Marcelo Carvalho dos
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Universidade Federal do Espírito Santo
Resumo
The aim of this study was to evaluate the efficacy of two solutions on the prevention of adhesion formation in ewes submitted to successive surgical embryo collection. Eight cross-bred ewes were superovulated with 250IU of FSH and embryo collection was performed on D7. In G1 ewes (n=4), immediately before abdominal wall closure, dexametasone (25 mg/kg) diluted in Ringer with lactate (q.s. 75mL) was infused in the abdominal cavity, and in G2 (n=4), vitamin E (100 mg/kg) diluted in olive-oil (q.s. 75mL). The animals underwent three surgical embryonic collections (M1, M2 and M3) with a range of about 60 days. Adhesions were evaluated for the amount, location, extent, vascularity and tenacity. Was also measured the degree of uterine exteriorization. Blood samples were taken for determination of serum fibrinogen, C-reactive protein and mucoprotein on days D-1, D3, D6 and D15, where D0 = day of surgery. There was no significant difference at 5% significance among groups and evaluations regarding the degree of adhesions and uterine exteriorization. Uterine exteriorization was possible in all animals and evaluations in G1 animals, but unable in 25% of G2 animals during the second embryo collection and in 75% of animals on third embryo collection and also at necropsy. Fibrinogen, mucoprotein and C reactive protein serum values did not differ between groups and days of observation. The average rates of embryo recovery were different only in M2 (P<0.04) between G1 and G2. It is concluded that dexametasone diluted in Ringer with lactate, in the dose and volume used, is efficacious in the prevention of abdominal adhesion formation in ewes submitted to successive embryo surgical collection and allows until four successive embryo collections
The aim of this study was to evaluate the efficacy of two solutions on the prevention of adhesion formation in ewes submitted to successive surgical embryo collection. Eight cross-bred ewes were superovulated with 250IUof FSH and embryo collection was performed on D7. In G1 ewes (n=4), immediately before abdominal wall closure, dexametasone (25 mg/kg) diluted in Ringerwith lactate (q.s. 75mL)wasinfused in the abdominal cavity,and in G2 (n=4), vitamin E (100 mg/kg) diluted in olive-oil (q.s. 75mL). The animals underwent threesurgical embryoniccollections(M1, M2 and M3) with a range of about 60 days. Adhesions were evaluated for the amount, location, extent, vascularity and tenacity. Was also measured the degree of uterine exteriorization. Blood samples were taken for determination of serum fibrinogen, C-reactive protein and mucoprotein on days D-1, D3, D6 and D15, where D0 = day of surgery. There was no significant difference at 5% significance among groups and evaluations regarding the degree of adhesions and uterine exteriorization.Uterine exteriorization was possible in all animals and evaluations in G1 animals, but unablein 25% of G2 animals during the second embryo collection and in 75% of animals on third embryo collection and also at necropsy. Fibrinogen, mucoprotein and C reactive protein serum values did not differ between groups and days of observation. The average rates of embryo recovery were differentonly in M2 (P<0.04) between G1 and G2.It is concluded that dexametasone diluted in Ringerwith lactate, in the dose and volume used, is efficacious in the prevention of abdominal adhesion formation in ewes submitted to successive embryo surgical collection andallows until four successive embryo collections.
The aim of this study was to evaluate the efficacy of two solutions on the prevention of adhesion formation in ewes submitted to successive surgical embryo collection. Eight cross-bred ewes were superovulated with 250IUof FSH and embryo collection was performed on D7. In G1 ewes (n=4), immediately before abdominal wall closure, dexametasone (25 mg/kg) diluted in Ringerwith lactate (q.s. 75mL)wasinfused in the abdominal cavity,and in G2 (n=4), vitamin E (100 mg/kg) diluted in olive-oil (q.s. 75mL). The animals underwent threesurgical embryoniccollections(M1, M2 and M3) with a range of about 60 days. Adhesions were evaluated for the amount, location, extent, vascularity and tenacity. Was also measured the degree of uterine exteriorization. Blood samples were taken for determination of serum fibrinogen, C-reactive protein and mucoprotein on days D-1, D3, D6 and D15, where D0 = day of surgery. There was no significant difference at 5% significance among groups and evaluations regarding the degree of adhesions and uterine exteriorization.Uterine exteriorization was possible in all animals and evaluations in G1 animals, but unablein 25% of G2 animals during the second embryo collection and in 75% of animals on third embryo collection and also at necropsy. Fibrinogen, mucoprotein and C reactive protein serum values did not differ between groups and days of observation. The average rates of embryo recovery were differentonly in M2 (P<0.04) between G1 and G2.It is concluded that dexametasone diluted in Ringerwith lactate, in the dose and volume used, is efficacious in the prevention of abdominal adhesion formation in ewes submitted to successive embryo surgical collection andallows until four successive embryo collections.
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Palavras-chave
Aderências abdominais , Colheita cirúrgica , Dexametasona , Ringer com lactato , Vitamina E , Azeite de oliva , Proteínas de fase aguda , Ovinos