Análises de padrões isoméricos de canabinóides e produtos de Cannabis por GC-MS, GCxGC-qMS e UPLC acoplado a espectrometria de massas de mobilidade iônica (TWIM-MS)
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Data
2018-02-15
Autores
Santos, Nayara Araújo dos
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Universidade Federal do Espírito Santo
Resumo
CannabissativaL. plant, popularly known as marijuana, is considered a complex matrix due to its many constituents, among them more than 100 are classified as cannabinoids. These compounds are distributed in the plant in different isomeric forms, which hinders the unambiguous characterization of the psychoactive compounds. Therefore, the present study reported the analysis of seven cannabinoid standards (five neutral: ?9-THC, CBD, CBC, CBN and CBG,and two cannabinoid acid standards: ?9-THCA A and CBDA), as well asCannabisproducts (hashish and marijuana) and parts of theCannabisplant (flower andleaf) using four different analytical techniques, mono and two-dimensional gas chromatography (GC-MS and GCxGC-qMS), Ultra performance liquid chromatography coupled to mass spectrometry (UPLC-ESI(±)TOF-MS) and ion mobility spectrometry (UPLC-ESI(±)-TWIM MS). The results obtained by GC-MS demonstrated extremely close retention times (?t=1.303 min) in the five cannabinoids analyzed from the use of a non-polar stationary phase, DB5. However, GCxGC-qMS resulted in a better distinction in the identification of cannabinoids constitutional isomers, where four of eleven cannabinoids were detected in the three samples; hashish, flower andCannabisleaf. The analysis, by UPLC-ESI(±)TOF-MS, of marijuana and hashish samples, allowed the identification of eight isomers in the XIC mode at m/z315.2354. In the analyzes by UPLC-ESI(-)TWIM MS, a largenumberof compounds was mainly detected for hashish and flower samples. Asfor cannabinoidsofm/z357, ten peaks could be detected,equally distributed, in flower and hashish chromatograms. Although GC is one of the most used techniques in the study of cannabinoids inCannabisand its products, it does not allow the identification of acid cannabinoids, which are widely available in the plant, without previous laborious preparatory stages. Differently, liquid chromatography combined with ion mobility mass spectrometry, allowed the identification of both types of cannabinoids (acids and neutrals), in addition to providing a greater detection of constitutional isomers compounds compared to classical GC technique
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Cannabinoids , Isomers , Mass Spectrometry , Isômeros , Canabinóides
Citação
SANTOS, Nayara Araújo dos. Análises de padrões isoméricos de canabinóides e produtos de Cannabis por GC-MS, GCxGC-qMS e UPLC acoplado a espectrometria de massas de mobilidade iônica (TWIM-MS). 2018. Dissertação (Mestrado em Química) – Universidade Federal do Espírito Santo, Vitória, 2018.