Aproveitamento de resíduos para produção de lipases fúngicas via fermentação em estado sólido
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Data
2018-02-27
Autores
Teodoro, Arthur Fagundes dos Santos
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Universidade Federal do Espírito Santo
Resumo
Lipases are enzymes responsible for the catalysis of reactions from a wide variety of industrial processes. Solid fermentation appears as an alternative for the production of enzymes due to the low cost and possibility of using residues of low value and with great abundance, besides requiring a smaller amount of energy in comparison to the submerged fermentation. This work had as objective to produce lipases by the fungi Penicillim sp. and Rhizomucor sp. by solid fermentation using sugar cane bagasse and coconut fiber. Initially, preliminary tests were carried out to test the use of a nutrient solution. After the tests, the enzyme production was optimized through an experimental design 33, where the influence of the inducer concentration (soybean oil) was studied in 2.5%, 5% and 10%, at a temperature of 27 ° C, 30 ° C and 33 ° C, and 40%, 60% and 80% field retention capacity for each medium and fungus. With the use of sugarcane bagasse as a support medium, Penicillium sp presented the highest lipase activity (147,5 U/g) at 30°C temperature, 60% field capacity and 5% of the inductor, with the Inductor Concentration and Temperature with significant influence on the results. Rhizomucor sp had the highest lipase activity (161,3 U/g) under the same conditions mentioned above and with the same significant influence on the results. For coconut fiber, Penicillium sp presented the highest lipase activity (75 U/g) under conditions of 30 ° C temperature, 60% humidity and 10% of the inductor, with field capacity, concentration of the inductor and temperature with significant influence on results. Rhizomucor sp had the highest lipase activity (78,9 U/g) under the same conditions mentioned above and with the same influence in the results.
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solid state fermentation , Lipases , Penicillium sp , Rhizomucor sp , Enzimas , Fermentação em estado sólido