Caracterização histopatológica e imunoistoquímica de bexigas de bovinos com hematúra enzoótica
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Data
2012-02-27
Autores
Silva, Maria Aparecida da
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Universidade Federal do Espírito Santo
Resumo
Bovine enzootic hematuria (BEH) is caused by chronic ingestion of Pteridium aquilinum and is characterized by the presence of blood in the urine and development of lesions in the urinary bladder and is responsible for economic losses. Poisoning by this plant can also occur in humans. The objective was to evaluate the lesions in bladders of animals with BEH in the south region of the Espírito Santo. For this, were evaluated 350 bladders of bovines in a slaughterhouse and, of these, selected 46 that had macroscopic lesions and/or hematuria. Samples of each bladder were fixed in formalin 10% submitted to histological processing and classified by histomorphology. The immunohistochemistry was performed with anti-vimentin, anti-cytokeratin, anti-CD31, anti-VEGF and anti-uroplakin only in the 26 bladders that presented neoplastic lesions. Non-neoplastic lesions were observed in 100% of samples and the neoplastic in 56.52%. The presence of tumors was significant (p<0.05) in the caudal portion of the bladder. Detected neoplastic types were urothelial carcinoma, in situ carcinoma, adenocarcinoma, hemangioma, myxoma and hemangiosarcoma. There was a higher frequency of dysplasia, clear cell metaplasia, inflammation and vascular thickening in bladders with neoplasm. The expression of cytokeratin was significant (p<0.05) in epithelial neoplasms and vimentin in mesenchymal neoplasms. Uroplakin III differed in varied types of neoplastic lesions and showed to be typical and atypical while that of CD31 was significantly (p <0.05) in vascular mesenchymal neoplasms. A significant difference (p <0.05) in the number of vessels extratumorais stained by VEGF between myxomas and adenocarcinomas, and in intratumoral vessels stained by CD31 and VEGF in the different tumor types. Positive correlation existed between the number of intra- and extratumoral vessels in hemangiomas, hemangiosarcomas, and myxomas stained by CD31; between hemangiomas, myxomas, and adenocarcinomas stained with VEGF; between the expression of vimentin and CD31 and between cytokeratin and uroplakin. It is concluded that bladders from bovines with BEH have non-neoplastic and neoplastic lesions, isolated or associated. Biomarkers aid in the differentiation of the histogenesis of epithelial and vascular mesenchymal neoplasms. Uroplakin demonstrated to be effective for the assessment of integrity urothelial, and vascular markers (CD31 and VEGF) for endothelial integrity and for prognosis
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Palavras-chave
BEH , Neoplasm , PECAM-1 , VEGF , Uroplakin , HEB , Neoplasia , PECAM-1 , VEGF , Uroplaquina
Citação
SILVA, Maria Aparecida da. Caracterização histopatológica e imunoistoquímica de bexigas de bovinos com hematúra enzoótica. 2012. 112 f. Dissertação (Mestrado em Ciências Veterinárias) - Universidade Federal do Espírito Santo, Alegre, 2012.