Ciências Odontológicas
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Navegando Ciências Odontológicas por Autor "Barroso, Danielle Resende Camisasca"
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- ItemAcurácia do dispositivo VELscope® para diagnóstico complementar de desordens orais potencialmente malignas (DOPMs)(Universidade Federal do Espírito Santo, 2023-12-19) Jesus, Eduarda Barboza Layber de; Barros, Liliana Aparecida Pimenta de; http://lattes.cnpq.br/8635277660080840; https://orcid.org/0009-0005-7083-4144; http://lattes.cnpq.br/4167117921403314; Barroso, Danielle Resende Camisasca; http://lattes.cnpq.br/3989074593181674; Lourenco, Simone de Queiroz ChavesThe early diagnosis of oral potentially malignant disorders (OPMDs) through visual inspection followed by histopathological confirmation is a fundamental step towards reducing the rates of lesions that have undergone malignant transformation. The use of the VELscope device has been suggested as an adjuvant tool through fluorescence visualization in the early detection of OPMDs. The aim of our study was to characterize the diagnostic accuracy of the VELscope in recognizing the autofluorescence of OPMDs, correlating clinical and histopathological aspects. Fifty-six oral mucosa lesions were submitted to conventional oral examination (COE) followed by VELscope and their autofluorescence characteristics were compared with the histopathological diagnosis. Sensitivity, specificity, positive and negative predictive values, accuracy and positive and negative likelihood ratios were calculated. The VELscope test showed sensitivity and specificity values of 50% and 69%, respectively, while the positive predictive value was 47.6% and the negative predictive value 71.4%. The overall accuracy was 62.5% and the positive and negative likelihood ratios were 1.63 and 0.72, respectively. The VELscope exam alone cannot provide a definitive diagnosis regarding the presence of dysplastic tissue changes and high-risk lesions. Despite having reasonable sensitivity, the high number of false-positive and false-negative results limits its effectiveness as an adjuvant, reinforced by the unfavorable negative likelihood ratio. A comprehensive and systematic conventional oral examination, biopsy and histopathological evaluation are the reference standards for the evaluation of suspicious lesions of the oral mucosa, considering the low accuracy of the VELscope device as an auxiliary method equipment in clinical examination.
- ItemAnálise histomorfométrica e da citotoxicidade de uma biocerâmica de fosfato de cálcio obtido a partir da casca de ovo de galinha para engenharia tecidual(Universidade Federal do Espírito Santo, 2023-06-14) Nascimento Neto, Conrado Dias do Nascimento; Silva, Daniela Nascimento; http://lattes.cnpq.br/3613001852759565; https://orcid.org/0000-0002-2097-5169; http://lattes.cnpq.br/0930321951875182; Barroso, Danielle Resende Camisasca; http://lattes.cnpq.br/3989074593181674; Maia, Monica Diuana CalasansGoal: To investigate the cytotoxicity and effect on tissue repair of a hydroxyapatite/dicalcium phosphate anhydrous (HA/DCPA) material obtained from chicken eggshell compared to the commercially available maxresorb® (MXR). Methods: Cytotoxicity was evaluated in fibroblasts (L cell, L-929) by the MTT test. Tissue repair was evaluated after grafting of biomaterials into critical-sized bone defects (CSDs) trephined in the calvarial bones of 24 Wistar rats. The animals were euthanized after 30, 60, and 90 days and the bone specimens containing the graft area were examined by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) (n=3), and histologic and histomorphometric analyses (n=21). The percentages of newly formed bone, connective tissue, remaining biomaterial, and total tissue repair area were compared between groups using Student’s t-test and ANOVA (p≤0.05). Results: HA/DCPA did not exhibit any cytotoxicity. Histologically, the CSDs contained newly formed bone from the defect margins and from ossification centers interspersed throughout the biomaterials. At 30 days, animals in the HA/DCPA group had a larger total tissue repair area than those in the MXR group (p=0.047), due to preservation of newly formed bone, connective tissue, and remaining biomaterial within the CSDs. No differences were observed between the experimental groups regarding percentage of newly formed bone, connective tissue, and remaining biomaterial at the time points of observation (p>0.05). Conclusions: Chicken eggshell-derived HA/DCPA is devoid of cytotoxic potential and can induce new bone formation and complete tissue filling of critical-sized bone defects with a degree of biomaterial degradation to the same extent as MXR.
- ItemComparação Clínicopatológica e da Expressão da Citoqueratina-10 Entre Liquen Plano e Lesão Liquenoide Oral(Universidade Federal do Espírito Santo, 2024-03-07) Moreira, Milena Duarte; Barroso, Danielle Resende Camisasca; https://orcid.org/0000-0003-1193-2113; http://lattes.cnpq.br/3989074593181674; https://orcid.org/0009-0002-2952-7474; http://lattes.cnpq.br/0214358029056111; Barros, Liliana Aparecida Pimenta de; https://orcid.org/0000-0002-1649-3116; http://lattes.cnpq.br/8635277660080840; Silva Júnior, José de Assis; https://orcid.org/; http://lattes.cnpq.br/9737483962693792Background: Oral lichen planus malignant transformation potential has been largely debated. Cytokeratin-10 is suggested as an indicator of a dysplastic epithelium and can be used to assess malignant progression in oral potentially malignant disorders. This study aimed to compare clinical, histopathological features and immunostaining for cytokeratin-10 between oral lichen planus and oral lichenoid lesion. Methods: Retrospective longitudinal study comparing lichen planus and oral lichenoid lesions diagnosed at the Oral Pathological Anatomy Service, analysing socio-demographic, clinicopathological data and CK-10 expression. Chi-Square, Fisher's exact test and Mann-Whitney or Student's ttests were used, when appropriate; and p-values <.05 were considered significant. Results: A total of 23 lichen planus and 23 lichenoid lesions were included. There was an association between oral lichen planus and symptomatology (p=0.031). The buccal mucosa was the most affected site in both groups, 20 cases (87.0%) in lichen planus, and 16 cases (69.6%) in oral lichenoid lesion. Bilateral (p<0.001) striae (p=0.004) are more characteristic of oral lichen planus. There was an association of oral lichen planus with degeneration of the basal layer (p=0.049), as well as with mild epithelial dysplasia (p<0.001). Cytokeratin-10 immunostaining was similar between groups. Conclusion: A continuous follow-up is necessary to identify different patterns of malignant transformation between groups of lesions, as well as a comparison with lesions with a higher malignant transformation rate.
- ItemEPIDERMÓLISE BOLHOSA NA SAÚDE ORAL: MANIFESTAÇÕES CLÍNICAS E ALTERAÇÕES SALIVARES(Universidade Federal do Espírito Santo, 2022-09-16) Azevedo, Brenda Lamonica Rodrigues de; Souza, Leticia Nogueira da Gama de; https://orcid.org/0000-0001-6330-996X; http://lattes.cnpq.br/2797885632539769; https://orcid.org/; http://lattes.cnpq.br/; Barroso, Danielle Resende Camisasca; https://orcid.org/0000-0003-1193-2113; http://lattes.cnpq.br/3989074593181674; Siqueira, Adriane Sousa de; https://orcid.org/0000-0002-4588-3906; http://lattes.cnpq.br/0657859298924213Epidermolysis bullosa (EB) is an inherited mucocutaneous disease characterized by fragility of the skin and mucous membranes with recurrent blistering. The four main types of EB are simple; junctional; dystrophy and Kindler syndrome. All EB patients can experience oral mucosal lesions such as erythema, ulcer, atrophy and blisters. However, the frequency, extent and severity vary from mild to severe depending on the type of EB. A sociodemographic survey, clinical assessment of the mouth cavity and analysis of aspects related to the saliva of individuals living with EB were carried out and who are followed up at the Blue Butterfly Project of Faculdades Integradas Espírito Santenses (FAESA) and at Hospital Nossa Senhora da Glória. A total of 11 patients (adults and children) diagnosed with EB were part of the sample, of which 27.27% were simple EB, 63.64% recessive dystrophic EB and 9.09% junctional EB. A frequency of 90.90% of patients with recessive dystrophic EB had lesions, while in the junctional EB group it was 9.10%. No patient with simple EB manifested lesions in the oral mucosa. The lesions were similarly distributed between the sexes and the most affected sites were the lips (54%), hard palate (36%) and buccal mucosa (27%). Ankyloglossia, ulcers, blisters and depapilated tongue were more frequently diagnosed. In salivary flow, the intragroup comparison revealed an increase in stimulated collection in relation to passive collection in control subjects (p = 0.0064). In the EB group there was no difference (p = 0.6086). We also did not observe differences in salivary volume between the control and EB groups (p = 0.7117 and p = 0.5557, passive and sti mulated flows respectively). Patients living with EB may have oral lesions, but the recessive dystrophic subtype had a severe clinical picture, with a high rate of mucosal lesions. Regarding salivary flow, it is not clear whether EB individuals are predisposed to manifest hyposalivation. The findings reinforce the need for routine dental follow-up in the multidisciplinary care of individuals living with EB.
- ItemLeucoplasia oral e leucoplasia verrucosa proliferativa: análise clínico-patológica e imunohistoquímica(Universidade Federal do Espírito Santo, 2023-12-18) Pereira, Luanna Canal; Barroso, Danielle Resende Camisasca; http://lattes.cnpq.br/3989074593181674; https://orcid.org/0000-0001-9582-7067; http://lattes.cnpq.br/8124717525751212; Lourenço, Simone de Queiroz Chaves; Barros, Liliana Aparecida Pimenta de; http://lattes.cnpq.br/8635277660080840Introduction: Oral potentially malignant disorders (OPMDs) are a group of oral lesions associated with a variable risk of progression to oral carcinomas. Oral leukoplakia (OL) is the most common OPMD. Proliferative verrucous leukoplakia (PVL) is a subtype of OL with a high risk of progression to carcinoma. Cytokeratin 10 (CK10) acts to differentiate normal from dysplastic epithelium. Objectives: Compare sociodemographic, clinical, histopathological and immunohistochemical characteristics of CK10 in OL and PVL and apply two diagnostic criteria for PVL. Materials and methods: Cases diagnosed as OL, PVL, epithelial dysplasia and hyperkeratosis without and with dysplasia were selected from the Oral Pathological Anatomy Service of the Federal University of Espírito Santo, in ten years. Sociodemographic, clinicopathological and immunohistochemical data were collected. A p-value ≤.05 was considered was considered statistically significant. Results: 51 patients (n=32, 62.7% LVP and n=19, 37.3% LO) and 104 lesions (n=76, 73% LVP and n=28, 26.9% LO) were selected, with 53 lesions submitted to immunohistochemical staining for CK10 (n=40, 75.4% LVP and n=13, 24.5% LO), with a mean follow-up of 13 months for LO and 27 months for LVP (p=0.038). There was a predominance of females in both, an association between tobacco use and LO (p= 0.007) and the presence of recurrence in PVL lesions (p=0.028). There was malignant transformation in two cases of PVL and one of LO. Almost all lesions were positive for CK10 (n=51, 96.3%). No lesion showed CK10 expression in the basal layer. When applying the criteria for PVL, all met the criteria of Cerero-Lapiedra et al., 2010 and 19 patients met the criteria of Villa et al., 2018. Conclusions: Tobacco use is associated with LO, but not with LVP. The two criteria applied were useful, CereroLapiedra et al., 2010 selects more patients, a favorable fact for better monitoring of the disease. There was no difference in CK10 expression between the lesions studied.
- ItemPrevalência e Fatores de Risco Associados à Colonização por Candida spp. em Lesões Da Mucosa Bucal(Universidade Federal do Espírito Santo, 2024-03-12) Feletti, Mariana Pires; Tavares, Sarah Gonçalves; https://orcid.org/0000-0003-4170-4445; http://lattes.cnpq.br/1018708169217296; Velloso, Tânia Regina Grão; https://orcid.org/0000-0001-6865-7955; http://lattes.cnpq.br/6427157237870625; https://orcid.org/0000-0003-2619-5953; http://lattes.cnpq.br/0978973634199704; Melo, Analy Salles de Azevedo; https://orcid.org/0000-0001-6900-6468; http://lattes.cnpq.br/8922840487452492; Barroso, Danielle Resende Camisasca; https://orcid.org/0000-0003-1193-2113; http://lattes.cnpq.br/3989074593181674The present study aimed to evaluate the prevalence of Candida spp. in lesions that affect the oral mucosa, assess the distribution of Candida species present, and analyze the risk factors associated with this relationship. A cross-sectional study was conducted in which patients with lesions on the oral mucosa underwent swab collection to investigate Candida spp. The first collection was carried out in the lesion area, and a second in the mucosa region without apparent lesion. Patient samples were plated on CHROMagar Candida™, and colony-forming unit counts were performed to determine fungal load. Biochemical, morphological, and mass spectrometry tests were carried out to identify the species, followed by in vitro sensitivity tests to antifungals. The data collected indicated a prevalence of 40% of Candida spp. associated with oral lesions in x patients evaluated. Among the most commonly found species, C. albicans (62.5%), C. glabrata (10.5%), C. parapsilosis (7.5%), and C. tropicalis (7.5%) stood out. It was observed that the presence of the lesion was a factor that influenced the occurrence of Candida spp., with a more significant occurrence of this microorganism in areas of altered mucosa. Furthermore, Candida spp. it was more prevalent in elderly, female, and diabetic patients. No association was identified between Candida spp. and lesions in the oral mucosa with hyposalivation, history of smoking, and alcohol consumption. Of the 37 samples submitted to antifungal susceptibility testing, all have a resistance profile to Nystatin. Based on the data from this study, it is concluded that lesions in the oral mucosa represent a risk factor for colonization by Candida spp. Although most species identified are C. albicans, nonalbicans species were isolated in the oral cavity of patients, emphasizing C. parapsilosis. Understanding the relationship between species and lesions in the oral mucosa is crucial for safer and more effective patient clinical management.