Mestrado em Biologia Vegetal

URI Permanente para esta coleção

http://repositorio.ufes.br/handle/10/17506

Nível: Mestrado Acadêmico
Ano de início: 2002
Conceito atual na CAPES: 4
Ato normativo: Homologado pelo CNE (Portaria MEC Nº 609, de 14/03/2019. Publicação no DOU 18 de março de 2019, seç. 1, p.52 - Parecer CNE/CES no 487/2018, Processo no 23001.000335/2018-51).
Periodicidade de seleção: Anual
Área(s) de concentração: Filosofia
Url do curso: https://biologiavegetal.ufes.br/pt-br/pos-graduacao/PPGBV/detalhes-do-curso?id=28

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Ecofisiologia de um trecho de mata seca de restinga ocorrente no Parque Estadual Paulo César Vinha, Guarapari (ES)
(Universidade Federal do Espírito Santo, 2004-12-10) Assis, André Moreira de; Thomaz, Luciana Dias; Leite, Idalina Tereza de Almeida; Santana, Reynaldo Campos
(Floristic survey of a restinga forest in Guarapari, Espirito Santo State, Brazil). The Paulo César Vinha State Park in Setiba, Guarapari Municipality (ES) is the best studied of all restinga conservation units in the state of Espírito Santo. But there is still much to be learned concerning the flora of some of the plant communities found here. From 1997 to 2000, plants were collected on random walks through approximately 4 hectares of forest that covers the outer beach ridges of the PCVSP. A total of 172 species were surveyed belonging to 54 families. The most species-rich families are Myrtaceae (25 spp.), Bromeliaceae (14), Orchidaceae (13), Sapotaceae (10), Lauraceae (07) and Rubiaceae (07).The most common geographic distribution pattern, based on the literature, was that of Atlantic coast species, followed by widespread species, speciesfound on both the Atlantic coast and in central Brazil, disjunct species and those endemic to Espírito Santo. Floristic similarity with other coastal areas is directly related to the distance between these areas. The presence of endemic and threatened species in the neighborhood of the Park reinforces the need for effective conservation of this area and provides the incentive to create other conservation units in the restingas of Espírito Santo state
Eficiência fotoquímica das plantas e atividade da enzima pectinametilesterase na polpa de frutos do mamoeiro (Carica papaya L.)
(Universidade Federal do Espírito Santo, 2005-02-17) Fontes, Renata Venturim; Figueiredo, Suely Gomes de; Silva, Diolina Moura; Balbino, José Mauro de Souza
This work was developed as objective to detect, by the technique of emission at the chlorophyll fluorescence, stress in mamoeiro plants of cultivates Tainung and Sunrise Solo, aiming at to improve the handling preharvest of the culture. The experiments had been lead in the field and, monthly, they had been made measured of emission at the chlorophyll fluorescence as well the text of nitrogen, match and potassium in petiole foliar and the activity of nitrate reductase in leaves. The values of amplitude of day/night temperature, maximum temperature and monthly rainfall of the region had been gotten of a metereological station at INMET in the Incaper/Linhares-ES. The presented results had allowed to conclude that high values of amplitude of day-night temperature had caused increase of the initial fluorescence and reduction of the efficiency photochemistry only in the plants of cultivate Tainung, that had also presented its photochemistry efficiency more sensible to the increase of temperature of that the plants of cultivate Sunrise Solo, that had presented its photochemistry efficiency more sensible to the rainfall variations. The nitrogen text in petiole foliar was presented higher how much bigger it was the text of match and potassium in petiole foliar in both cultivates Tainung and Sunrise Solo. The activity of the enzyme nitrate reductase increased with the nitrogen text in plants of both cultivates. The values of correlation, between photochemistry efficiency and activity of nitrate reductase, presented for cultivates them, had shown that the photochemistry efficiency in the plants of cultivate Sunrise Solo is more dependent the activity of nitrate reductase that the photochemistry efficiency of the plants of cultivate Tainung. Based in the results, it is concluded that the photochemistry efficiency of fotossistema II, gotten by means of the technique of emission at the chlorophyll fluorescence, can be used as tool to indicate stress in mamoeiro plants, aiming at to improve the handling in the preharvest of the culture.
Respostas fisiológicas e bioquímicas de Rhizophora mangle L. e Laguncularia racemosa Gaerth. à população ambiental no manguezal do município de Vitória, ES
(Universidade Federal do Espírito Santo, 2005-02-17) Falqueto, Antelmo Ralph; Figueiredo, Suely Gomes de; Silva, Diolina Moura; Lopes, Nei Fernandes; Cassini, Sérvio Túlio Alves
This research was done in order to evaluate the effects of environmental alterations on photosynthesis in Rhizophora mangleL. and Laguncularia racemosaGaerth. young plants. The data were collected during the rain and drought periods in four points atmangrove ofVitoria, ES. The obtained results indicated that the increase in absorption of iron and zinc for L. racemosaduring the dry period favored the reduction of initial fluorescence (Fo). Decreases Foindicate protection of photosynthetic machinery against damage generate by excess of energy excitation, which reflex immediately in the increase to photosystem II photochemical efficiency (PSII), Fv/Fm, in L. racemosa in dry period. The elevated values of Fv/Fmratio observed in thepointnumber 2during the rain period, in both species, constitute good indicators in thenitrogen concentration, which reflex the degree of contamination by pollution threw awaydirectly in the mangrove ecosystem. In the points of higher environmental pollution, has been observed higher use efficiency of ATP and NADPH (Ft) produced during the photochemical phase of photosynthesis, increasedof the Calvin cycle reactions velocity (Fm/Ft) and the CO2fixation ratio (Tdf) in R. mangle and L. racemosa, indicating increase to tolerance thesespecies to environment variations through to increase of photosynthetic rate
Algas fitoplanctônicas na Lagoa Juparanã (Linhares-ES): variação espacial, temporal e bioindicadores do estado trófico
(Universidade Federal do Espírito Santo, 2005-02-24) Gonçalves, Monica Amorim; Fernandes, Valéria de Oliveira; Dias Júnior, Camilo; Suzuki, Marina Satika
The present work was done at Juparanã lake –Brazil’s larger lake in fresh water volume –located in Linhares-ES. There were four collections with quarterly periodicity along an annual cycle (season drought and rainy), in two sampling stations: point 1, situated in central point (in the middle of the lake) and, because of this, more natural considered and less impact and point 2 , located in the outlet river Pequeno, that connects the lake to the river Doce. Characterist limnological like, temperature, dissolved oxygen, pH, water electric conductivity, depth, main nutrients concentration, transparency were determined. The phytoplankton community was analysed in qualitative and quantitative terms, being made thecounting by the sedimentation method, besides the chlorophyll a determination.The obtained results showed that the lake can be characterized for pH with tendency to the neutrality (6,5), low electric conductivity (84,8 µS/cm), low turbidity (12,1 UNT) and nutrients poverty (PO43 -34,0 µg/L; NO3-118,6 µg/L; P-total 42,2 µg/L; NH4+48,9 µg/L; NO2-4,5 µg/L; N-total 550,7 µg/L), water transparency was high in the two first samplings, but it decreased considerably in the next samplings due to the rainfall season, the dissolved oxygen contents were elevated during the whole study and wasn’t verified thermal profile stratification most of the time. Phytoplankton community showed 109 algaes taxa, belonging to 9 algaes classes with low numerical density (794 ind./ml) and biomass algal numbers (1,4 µg/L of chlorophyll a). Cyanophyceae, Zygnemaphyceae and Chlorophyceae was dominant numerically. Zygnemaphyceae were represented mostly for algaes of family Desmidiaceae, described in the literature like bioindicators of oligotrophic waters. The most representative gender regardind taxa number was Staurastrum. Chlorophyceae represented mostly by Chlorococcales, and Eutetramorus/Radiococcuswas abundant in 50% of the quantitative samples. Regarding to Cyanophyceae although low density, Synechocystissp. was dominant in 22,7% of the samples and abundant in 54,5%. The Simple and Compound Nygaard Coefficient classifies as an mesotrophic to eutrophic ambient, however this index works with big taxa groups was not considered satisfactory in bioindicators studies. In point 2 were found filamentous species that usually are part of periphyton, very developed in this location. There wasn’t observed standards in phytoplankton vertical and temporal distribution. The low concentrations of nutrients and low levels of biomass and phytoplankton density points to oligotrophic conditions for the Juparanã lake and suggests to possible competitive relations between phytoplankton, periphyton and aquatic plants, mostly in point 2.Qualitative and quantitative analysis demonstrate Cyanobacteria's several taxa,with dominanceof Synechocystissp., what is an alertfor the pond uses, due to the toxins production potentiality.
Variações da comunidade fitoplanctônica na região estuarina dos rios Piraquê-Açú e Piraquê-Miriam (Aracruz, ES) e suas relações com os fatores ambientais
(Universidade Federal do Espírito Santo, 2005-02-25) Schaeffer, Jane Karla; Dias Júnior, Camilo; Suzuki, Marina Satika; Fernandes, Valéria de Oliveira
This study was carried out in the estuary of the Piraquê- Açú and Piraquê-Mirim rivers, located in the municipal district of Aracruz-ES (Brazil). The aim of this study is to verify both spatial and temporal changes on phytoplanktonic community of the estuary in its qualitative and quantitative aspects, and also to evaluate the biomass through the technique of biovolume. Moreover, the obtained biotics variables were related with abiotic variables analized at the same spots along the estuary. The obtained results were based on samplings carried out bimonthly in the period of August 2003 to February 2004, emphasizing two pluviometric seasons: dry (August) and rainy (February). Pheopigments concentrations were higher than chlorophyll a concentration, showing a senescent degree of the community. The qualitative aspect of the phytoplankton community showed 156 taxons classified in species, genus and varieties, wich most of them considered as marine, eurialine and planktonic. The dominant classes were Bacillariophyceae (57%), Chlorophyceae (11,5%) and Dinophyceae (8,4%). Although, the community showed a higher diversity, just some were quantitatively significant, among them Cyanophyceae Synechocystis aquatilis, Chlorophyceae Chlorella minutissima and Bacillariophyceae Melosira varians. The highest densities of total phytoplankton occured in the rainy season. Such variations were related to a significant contribution of allochthonous nutrients and materials originated from superficial outflow during the rainy season. Regarding the biovolume, seven classes of algae showed significative contribution: Chlorophyceae, Bacillariophyceae, Cyanophyceae, Dinophyceae, Cryptophyceae, Euglenophyceae and the phytoflagellates. The most significant contribution for total biovolume was given by Bacillariophyceae and Dinophyceae. Pluviosity was the major external driving force leading to changes in phytoplankton community
Indução de sistema de defesa do mamoeiro como resposta à elicitores químicos (óxido nítrico) e biológico (Saccharomyces cerevisiae)
(Universidade Federal do Espírito Santo, 2005-02-28) Santos, Mirella Pupo; Ventura, José Aires; Fernandes, Patricia Machado Bueno; Sabino, José Mauro de Souza; Santos, Reginaldo Bezerra dos
The yeast extract S. cerevisiae and nitric oxide has been studied in the induction of not specific answers in the defense in plants. Papaya plants were treated if SNP and yeast extract. The yeast extract in Golden culture induced increase of the free phenol concentration (39 %) and connected (35 %) after 72 hrs the treatment. In Sunrise Solo, the treatment induce the accumulation of connected phenols, the increase (30 %) occurred mainly 120 hrs after. The SNP was capable to increase in 63 % after 72 hrs the concentration of free phenols in Golden culture. In the Sunrise culture has increase of free phenols 30% after 72 hs. The treatment with the leavening induced the increase of the enzymatic activity in the Golden culture it was of 84% after 72 hrs, reaching at 336 % in 168 hrs. However in the Sunrise culture the increase in the activity of peroxide was of 87% and 225% after 72hrs and 168hrs respectively. The activity of peroxides in the Golden culture presented an increase of 129 % after 72 hrs and 219 % after 168 hrs after the treatment with SNP. In the Sunrise Solo culture it had an increase of 131% and 203 % after 72 hrs and 168 hrs respectively. In the Golden cultivar's has increase in the concentration of total sugar (46%) after 24 hs. In the cultivar Sunrise Solo after 24 hs has increase (24%) after yeast treatment. It had an increase in the concentration of sucrose in both culture when treated with leavening extract, been that this increase was bigger after 24 hrs and 48 hs of treatment (47% and 34%). The treatment with SNP induced an increase in the levels of sucrose in both cultures been that this increase was bigger (77%) after 24 hrs of treatment. The treatments with extract of leavening and SNP had caused an increase of the expression of the responsible gene for the production of peroxides on the production of lignin after 4 and 8hrs. The gene npr1 and gene sod-Mn did not have its expression modified with both treatments
Avaliação das características físicas, bioquímicas e organolépticas e efeito da radiação gama na conservação pós-colheita dos frutos de mamão (Carica papaya L.)
(Universidade Federal do Espírito Santo, 2005-02-28) Ferraz, Karla Kristine Florenço; Silva, Diolina Moura; Figueiredo, Suely Gomes de; Medeiros, Edna Faria de; Rabelo, Flavia Lucia Abreu
Avaliação da resistência à antracnose em frutos de diferentes genótipos de bananeira
(Universidade Federal do Espírito Santo, 2005-02-28) Alves, Eliomara Sousa Sobral; Fernandes, Patricia Machado Bueno; Ventura, José Aires; Costa, Hélcio; Chiaradia, Ana Cristina Nascimento
In Brazil, the banana culture has a great economical importance. It usually has a family character in Espírito Santo state, being produced in small farms. However, both pre-and post-harvest diseases have caused severe crop-lost. Yellow and black-sigatoka and panama disease can affect the plants still in the field. After the harvest, the fruits can be infected by the Colletotrichum musaeresponsible for the anthracnose in bananas. The choice of resistant genotypes for those diseases is a viable strategy; they can be achieved through improvement programs. In this work the resistance of different bananas genotypes that showed pre-harvested resistance (‘Prata’; ‘Pacovan’; ‘Ouro da Mata’; ‘Prata Zulu’; FHIA01; PV 42-68; PV 42-81; PV 42-142; ST 12-31; ST 42-08; YB 42-21), against the anthracnose were evaluated, using the ‘Prata’ cultivar as control. Additionally, chemical and physical features of the resistant fruits physiology were determined. For the resistance analysis and the genotypes characterization it was used ten and three repetitions, respectively. Statistical variance was measured by 5 % Tukey test. Four hybrids (ST 12-31; PV 42-68; PV 42-81; PV 42-142) were more resist to disease confirmed by small lesion diameter and pulp preserved. ST 12-31, PV42-81 and PV 42-142 had higher weight values than control, being 150,7g, 187,8g and 243,5g, respectively. The values for pulp weight vary from 72,4g (control) to 138,5g (PV 42-142) and the peel/pulp ratio from 1,6 (control) to 3,9 (‘Prata Zulu’). The fruit size varies from 2,6 cm (control) to 3,59 cm (‘Prata Zulu’) in diameter and 13,6 cm (control) to 19,95 cm (PV 42-142) in length. The peel thickness values varied from 1,7 mm (‘Prata Zulu’) to 4,6 mm (PV42-142), different of control (3,0 mm). The biochemical analysis showed perceptual values 0,36, 0,69 and 0,63 for total titulable acids (ATT) presented in PV 42-68, ‘Ouro da Mata’ and control, respectively. The genotypes YB 42-21, ST 42-08 and ‘Prata’ had 4,53, 4,52 and 4,28 pH values, respectively. The total soluble solids (SST) vary from 24,8 % (control) to 27,4 % (‘Prata Zulu’) and the ATT/SST ratio vary from 38,72 (control) to 67,56 (‘Prata Zulu’). For starch meaning, we did not found variation among ‘Pacovan’, PV 42-68 and ‘Ouro da Mata’, although all of them had highest values than control. Among the evaluated genotypes, PV 42-142, PV 42-68, PV 42-81 and ST 12-31 self showed more attractive to be used for agriculture practice and they can be recommended for the small farms. The results presented are the primer steps for future improvement programs
Estudo ecofisiológico de Schinus terebinthifolius Raddi (Aroeira Vermelha)
(Universidade Federal do Espírito Santo, 2005-03-17) Siqueira, Maria Carolina Nunes; Leite, Idalina Tereza de Almeida; Cuzzuol, Geraldo Rogério Faustini; Santos, Débora Leonardo dos
Schinus terebinthifolius(Raddi) is a widely distributed species, commonly called “pimenta-rosa”. The seed origin studies detects a genetic variability within the species, in this, within the same species, seeds originated from different locations are subjected to climatic ( and environmental) variations, that result in bring out certain aspects of its genetic composition, that means, that environment of a certain location can cause the development of specific characteristics, that will not manifest in a different location.Plants can react to environmental stress in different manners, and the toleranceto stress is variable among species and even in the same species depending on the habitat. This is the so-called phenotype plasticity. Studies which analyses forest species seedling responses to environmental stress are very important, since they allow theunderstanding of development process of these species under different abiotic conditions, which can influence the production and future generations.This work investigated S. terebintifoliusseed germinationfrom two different habitats:one from the “Restinga” (R) habitat and the other from wet forest (FP)under theseabiotic conditions: light, temperature, water stress and osmotic stress,and seedling developmentunder water stress. Seeds from both environments germinated under light and dark conditions. Seed water content from both populationswas about 21 and 25%, with no statistical difference between them. The FP populationhad higher germination percentage (69%), compared with R (41%), with a statistical difference between them. Under water and osmotic stress, the FPpopulationshowed a better germination compared to R. The results suggest that seed physiology is related to their original environment. Seeds are probably expressing genetic features in response to environment.Seedlings were grown from two different seed population. They were submitted to water stress for a 4 months period, and their development was recorded. The results showed that the stress affected seedling development, from the two populationsorigin. However, seedlings from FP populationshowed a higher development in relation to those from R, mainly in the most stressing treatments. These results suggests to a higher adaptation potential to seedlings from FP population
Influência da pressão hidrostática em Saccharomyces cerevisiae: correlação com estresses químicos e físicos
(Universidade Federal do Espírito Santo, 2005-03-31) Soares, Fernando Lucas Palhano; Fernandes, Patricia Machado Bueno; Batitucci, Maria do Carmo Pimentel; Foguel, Débora
Yeasts are unicellular organisms that are exposed to a highly variable environment,concerning the availability of nutrients, temperature, pH, radiation, access to oxygen and,specially, water activity. Evolution has selected yeasts to tolerate, to a certain extent, theseenvironmental stresses. High hydrostatic pressure (HHP) exerts a broad effect upon yeastcells, interfering with the cell membranes, cellular architecture and in processes ofpolymerisation and denaturation of proteins. Gene expression patterns in response to HHPrevealed a stress response profile. The majority of the upregulated genes are involved in stressdefence and carbohydrate metabolism while most of the repressed ones are in cell cycleprogression and protein synthesis categories (Fernandes et al, 2004). Nitric oxide (NO) is asimple and unique molecule that has diverse functions in organisms, including intracellularand intercellular messenger. The influence of NO on cell growth of Saccharomyces cerevisiaeand as a signal molecule in stress response of HHP was evaluated. Respiring cells were moresensitive to an increase in intracellular NO concentration than fermentatively growing cells.Low levels of NO demonstrated a cytoprotective effect during stress from HHP. Induction ofNO synthase was isoform-specific and dependent on the metabolic state of the cells and thestress response pathway. These results support the hypothesis that an increase in intracellularNO concentration leads to stress protection against HHP. In addition, the acquisition oftolerance to high hydrostatic pressure of 220 MPa (HHP) in response to a 0.4 mM hydrogenperoxide, 6 % ethanol or 10 ºC cold shock pretreatment for different lengths of times wasstudied in the yeast S. cerevisiae. The protection conferred by these different treatments wassimilar, around 3 log cycles and time-dependent. Analysis of the induction of the mostpressure up-regulated genes under these conditions was investigated by RT-PCR. Our resultsrevealed that the cell stress response to HHP shares common features with hydrogen peroxideand ethanol stresses, but differs in some way to cold shock. Also, it was seen that mildpressure induced cell cycle arrest and protection against severe stresses, such as hightemperature, high pressure and ultra cold shock. Nevertheless, this protection was only significant if the cells were incubated at atmospheric pressure after the HHP treatment.Expression of genes that were upregulated by HHP and are related to resistance to thisstresses were also analysed, and, for the majority of them, higher induction was attained after15 min post-pressurization. Taken together, the results imply an interconnection amongstresses.
Avaliação fisiológica e anatômica dos efeitos da deposição de ferro e dióxido de enxofre em duas espécies vegetais ocorrentes na restinga : Schinus terebinthifolius e Mimusopsis coriacea
(Universidade Federal do Espírito Santo, 2005-04-29) Lopes, Sandrelly Amigo; Cano, Marco Antonio Oliva; Thomaz, Luciana Dias; Cuzzuol, Geraldo Rogério Faustini
The objective is to analyze the effects of the settling of iron particles and of sulphur dioxide on the growth, on the physiological parameters and morphological and histo-chemical parameters, aiming at active bio monitoring. Schinus terebinthifolius (Anacardiaceae) and Mimusopsis coriacea (Sapotaceae) seedlings were exposed during an eight-month period to the gas and particle emissions of a pelletizing plant located in the municipal region of Anchieta, State of Espirito Santo. The plant species were selected because of what they represent in the lowlands environment, and because they are used for the purpose of planting trees in urban areas. These species’ uncomplicated planting care, as well as their morphological, phonologic and physiological characteristics, were also taken into consideration. Two experiments were conducted. In the first experiment, the species were placed in standard stations and spread throughout a transect according to the predominant wind direction in the region and at different distances: 200 meters, 500 meters, 800 meters, 1,400 meters and 1,700 meters away from the source of the emissions. The second experiment focused on temporal evaluation conducted at a station located in a product stocking and handling yard and in a reference area. Analyses were made of the trees’ height, the number of leaves, and the number and percentage of dead leaves, the foliar phyto-toxic chlorosis index, the foliar temperature, the temperature-related difference and the air, and the sedimentary dust content. The following data was analyzed on the last data gathering date: the settling of iron on the leaves, the iron and the sulphur content of the leaves, stems and roots, the chlorophyll a and b content, the gas exchange and the total dry mass of the plant material. In the second experiment, these analyses were conducted monthly, and included the number of flowering or non-flowering events, and the number of fruit or branches with fruit. The highest deposits of iron particles were verified at 200 meters. A higher quantity of settled iron particles per leaf area unit was observed at this same distance in the two species, S. terebinthifolius and M. coriacea. The highest foliar contents of total iron were verified at 200 meters in both plant species; that is, at the station located closest to the pelletizing plant, showing a gradual reduction as the distance from the emission source increased. The highest iron contents were found in the roots of both species. The highest content of total sulphate was observed in the M. coriacea, at 200 meters and in the S. terebinthifolius at 500 meters away from the emission source. There is a positive correlation between the foliar area and the settling of iron. No height-related variations were verified in the two species. The percentage of dead leaves was verified only in the S. terebinthifolius species lying at 500 meters and 800 meters away from the source. The number and the percentage of dead leaves and the necrosis phyto-toxicity index in the two species increased as exposure time to the plant’s emissions was extended. The same event was verified in the M. coriacea species lying 800 meters and 1,400 meters away from the source. A higher necrosis-related phyto-toxicity index of the M. coriacea was verified in the exposed plants located 200 meters and 1,400 meters away, whereas the highest such index for the S. terebinthifolius species was verified in the plants located 1,400 meters away. The pigment content of both species did not change because of the distance from the emission source. However, a slight reduction in the content of a and b chlorophyll and in the carotene content was verified at 800 meters. In general, the gas exchanges, that is, the reduction of the stoma conductance of the 11 transpiration and the liquid assimilation of CO2 in the S. terebinthifolius and M. coriacea, were lower at the stations located next to the plant. In other words, the related levels were generally lower at the stations lying closer to the emission source. M. coriacea proved to be more sensitive, with symptoms of damage to fluorescence at 200, 500 and 1,400 meters, whereas in relation to S. terebinthifolius, reduced damage was evidenced at 500 meters. In relation to the histo-chemical test, S. terebinthifolius accumulated higher amounts of iron with higher settling at the reentering angles at the bases of the uni-serial glandular trychomas, while M. coriaceae had a lower iron content, probably because of the leaves’ arrangement and smooth texture, the stomas hollows, the existence of thick hypodermis and cuticles on both sides of the leaves, acting as protection mechanisms to prevent the entry of polluting agents. As for the second experiment, there was higher settling of particles on the surface exposed to industrial activities conducted at the product stocking and handling yard, in contrast to the surface that had not been exposed. The exposed plants contained higher settlement and accumulation of iron in the case of both species. The settling and accumulation of iron increased in the S. terebinthifolius species during the exposure period, as did the number of this species’ leaves. This increase was verified to a greater extent at the station located in the industrial yard. This was not evidenced in relation to M. coriacea. Settling was reduced because of the rainy season. As for iron absorption, there was an increase, in general terms, for a period of two months after exposure to the facility. The figures related to iron accumulation in the plants exposed to the industrial area were higher for both species. Most of the accumulated iron was verified mainly in the roots of both species. A general increase in the content of plant species material was verified in both species. The growth in this material in S. terebinthifolius was verified in the industrial yard, while this growth in M. coriacea was verified in an area that had not been exposed to atmospheric emissions. There was no temporal variation in the height of the M. coriacea at the two stations, whereas there was more pronounced exponential growth of this material in the S. terebinthifolius located at the industrial yard. The quantity of flowers and fruit was significantly reduced on plants exposed to industrial conditions. The incidence of damage, measured according to the percentage of necrosis and to the phyto-toxicity index, was higher in the areas exposed to industrial emissions. Damage increased gradually in both species in relation to exposure time. There was no variation in the chlorophyll or in the carotenoids. The figures related to transpiration and stoma conductance in general were higher in the exposed areas. S. terebinthifolius and M. coriacea had higher CO2 assimilation rates throughout the exposure period and on the non-exposed area. Fluorescence for the two species was more affected during the month of August, probably as a result of the iron that had been absorbed and of the accumulation of iron particles in July, which were absorbed in the roots as a result of the rainfall in that month. According to histo-chemical tests, the species accumulated more iron on the exposed area and the S. terebinthifolius species accumulated higher quantities of iron than the M. coriacea did because of the former species’ morphological and anatomical characteristics, as mentioned above. M. coriacea was the more sensitive of the species. Both species are reaction bio indicators, and can be used in active bio indication. S. terebinthifolius is a resistant species that can be used in the reclaiming of areas damaged by mining activities because of its rapid growth rate and gene-typical plasticity.
Estudo de óleos essenciais extraídos de plantas medicinais, no controle do fungo Colletotrichum gloeosporioides causador da doença antracnose no mamão
(Universidade Federal do Espírito Santo, 2005-06-07) Ferreira, Wilson Soares; Fernandes, Patricia Machado Bueno; Santos, Reginaldo Bezerra dos; Schmildt, Edilson Romais; Sabino, José Mauro de Souza
Brazil is the world’s greatest papaya producer, being Espírito Santo the second producer of Brazil, first in productivity and the greatest exporting. The papaya’s cultivation does matter to social and economic areas, for create jobs, fixing the man in the country, for stimulate many properties economically besides to produce wealthy to country. The papaya’s disease brings economic lost, either with fall of productivity or either with the acceptation of the product for the consuming market. Among the principals diseases after-harvest stands out the anthracnose, caused for fungus. Considering the problem of wastes from chemical control in the fruits, becomes important the inquiry of alternative methods of control, with attention to those that do not cause ambient impact and do not harm the consumers’ healthy. Studies are being carrying out with the medicinal plants in the alternative agriculture. In this work fourspecies of medicinal plants had been considered: Lippia Alba(erva-cidreira), Cymbopogon citratus(capim-limão), Cymbopogon nardus(capim-citronela) e Aloysia triphylla(cidrão). These plants were cultivated in two different enviromment, one of them withfertilization and others care’s cultivation, while the other in which plants were cultivation naturally, without human intervention. It was extracted, through distillation of drags of vapor, the essential oils from leafs of these plants, in different months. Through of chromatography gas technique that identified the main chemical components of these oils essential. The plants L. alba, C. citratuse A. triphyllahad presented the monoterpene citral as the principal component of them essential oils, while the C. narduspresented as the principal component of its essential oil, the monoterpene citronelal. To assessment of fungicidal activity from essentials oils of these plants, were realized tests in vitro of inhibition of mycelia growth and the germination of conidia. All the extracted essential oils of these plants in study, demonstrated fungicidal potential, because they had inhibited efficiently the mycelia growth and the germination of conidia from fungi. Among the months where the extractions had been made stands out the month of June, for have been the month in which all the plants had supplied to greater amounts of essentials oils. The fertilization and others cares’ cultivation did not increase the production of oils, the comparative production when the plants were cultivated naturally. Among the four plants studied, a C. nardus, presented a percentile greater in the essential oil production in every month that it was collected, being therefore indicated to be cultivated objectifying these essential oils for the control of anthracnose
Estudo do metabolismo fotoquímico nas folhas e perfil da atividade da celulase e b-galactosidase nos frutos de Carica papaya L., cultivares Golden e Gran Golden
(Universidade Federal do Espírito Santo, 2006-02-08) Gallon, Camilla Zanotti; Figueiredo, Suely Gomes de; Silva, Diolina Moura; Sabino, José Mauro de Souza; Aguilar, Marco Antônio Galeas
This study focused the photochemical metabolism of leaves and the pattern of activity of the cellulase and ß-galactosidase enzymes in the Carica papaya L. fruit, Golden and Gran Golden cultivars. The content of chlorophyll was taken with a portable chlorophyll measuring device (SPAD 502) and the kinetics of the emission of fluorescence from chlorophyll awith a portable fluorometer, Handy PEA (Hansatech Instruments Ltd., King’s Lynn, Norfolk, UK). The leaves, after previously going through a thirty minute adjustment to the dark, underwent a flow of photons at 680 µmol m-2 s-1. The amount of rain and the temperature of the area cultivated were taken into account when analyzing the data. The polyphasic curve of the fluorescence of chlorophylla for both cultivars of papaya during the time frame of the study demonstrates that there was not a significant difference among phases O, J, I and P. Differences in the parameters of the energy flow by cross-section of the photosystem II were observed. The Golden cultivar showed a higher rate of absorption of excited energy (ABS/CS), capture of excited energy (TRo/CS) and transport of that energy (ETo/CS). These results suggest that the Golden cultivar showed more efficiency than the Gran Golden cultivar when utilizing luminous energy in the period with the highest level of precipitation. However, Golden cultivar also showed a higher rate of dissipation of energy (DIo/CS) reflected in an increase of initial fluorescence (Fo), considered an indication of stress in plants, and the lessening of the quantum efficiency of the photosystem II. The Gran Golden cultivar showed an increase in the capture of energy by cross-section (TRo/CS) and the transport of that energy (ETo/CS). This increase may have contributed to this cultivar presenting greater quantum efficiency of the photosystem II in the period of lowest precipitation levels. The analysis of the cultivated fruit was conducted in the Laboratory of Protein Chemistry of the Federal University of Espirito Santo (in the XIIPortuguese acronym, UFES). The action of the enzymes cellulase and ß-galactosidase during ripening was observed in the 8 days following harvest and analysis of pulp firmness and loss of water were also conducted. The percentage of water lost in the fruit was calculated according to the loss of fresh mass and the percentage of the loss of firmness was obtained with the aid of a penetrometer. The activities of the enzymes cellulase and ß-galactosidase were previously standardized for the papaya tree and then measured for the obtainment of the relation with the loss of water and the changes in the firmness of the pulp. The results showed that the greatest loss in firmness in the fruit of the Golden and Gran Golden cultivars occurred between the 3rd and the 4th days after the harvest and that the fruit of the Gran Golden cultivar lose pulp firmness faster than those of the Golden cultivar. These results confirm a visual observation reported by papaya growers, and that is that the fruit of the Gran Golden cultivar have a shorter post harvest period. Observation showed that cellulase activity was greatest on the 5th day after harvest in the fruit of the Gran Golden cultivar and on the 6th day after harvest in the fruit of the Golden cultivar. The activity of ß-galactosidase increased in both cultivars until the 5th day after harvest, but in the Gran Golden cultivar the activity of this enzyme was higher in the post harvest period. These results illustrate the role these enzymes play in the decrease of firmness, evidencing the need for further study of the factors that interfere in the activation of these enzymes in order to allow the understanding of the physiology of the papaya
Comunidade fitoplanctônica e variáveis limnológicas no Reservatório Rio Bonito - Rio Santa Maria da Vitória (Santa Maria de Jetibá - ES)
(Universidade Federal do Espírito Santo, 2006-02-20) Rubim, Karine Toso; Azevedo, Sandra Maria Feliciano de Oliveira e; Fernandes, Valéria de Oliveira; Dias Júnior, Camilo
This search was realized in the Rio Bonito reservoir - Santa Maria da Vitória river, situated 60 km far from Vitória, in the city of Santa Maria de a Jetibá – ES (Brazil). This reservoir has area of the 2,2km2 , maximum lenght of 14 km, maximum volume of 26.350.103 m3 and takes the generation of electric energy as a principal finality. The main study’s objective was to verify both spatial and temporal changes on phytoplanktonic community of the Rio Bonito reservoir and to relate with abiotic variables. The samplings were carried out in two stations (E1 and E2) of reservoir’s lacustrine area, in three different deeps (sub-surface, eufotic zone, and afotic zone), with trimonthly periodicity, on july/2004 to april/2005. Were analysed: climatological variables, air and water temperature, pH, total alkalinity, dissolved oxygen, transparency, turbidity, ammoniacal nitrogen, nitrite, nitrate, ortophosfate, chlorophyll a, feofitin, total density and of phytoplankton Class, specific diversity and equitabilty, taxon’s wealths, abundant and dominant species, qualitative and quantitative analysis of the toxins produced by cyanobacteria (microcystin, cylindrospermopsin and saxitoxin) in seston’s samples. How about the qualitative composition of phytoplankton community 106 taxon’s were registered, the Chlorophyceae Class was the most representative about taxon’s wealth, on both stations of sampling. There happened an elevated variation of the total numerical density of phytoplankton community, oscillating of 7.022 cells/mL (E1- afotic zone - january/05) to 905.674 cells/mL (E1 - sub-surface - july/04). How about the density there was a quantitative domain of Cyanophyceae Class, in a percentage that varied from 83,8% to 99,9%, the dominant specie on the three initial sampling periods was Cylindrospermopisis raciborskii and on the last period Synechocystis sp. Seven abundant species were found, wich are: Cylindrospermopsis raciborskii, Synechocystis sp, Synechococcus sp, Monoraphidiopsis sp, Oscillatoria jasorvensis, Pseudoanabaena sp - of the Cyanophyceae Class e Ankistrodesmus sp of the Chlorophyceae Class. Low values of specific diversity and equitability were registered because of the domination of two species on the studied period. The biggest concentrations of chlorophyll a were registered in the sub-surface and eufotic zone, coinciding with the depths with larger total density of phytoplankton. The rainfall informations confered two distinct periods, one with lower tax of precipitation (july to october/04) and the other with a bigger tax of precipitation verified from november to may /05, with atypical rains on may. The highests mediums of total numerical density were registered on july/04, and the lowers values were found on april/05, month that suceeded the high tax of precipitation. The analysis of main components showed that positive correlation between total numerical density of phytoplankton, especially Cyanophyceae Class, pH, dissolved oxygen, chlorophyll a in contraposition to depth collection, the concentrations of nitrate and to diversity. None of the tested toxins (microcystin, cylindrospermopsin and saxitoxin) was detected in the samples of seston. Although the Rio Bonito reservoir has not been constructed with the purpose of public water supply, the river Santa Maria da Vitória is used to this finality in Grande Vitória’s region. This reservoir is an imminent danger, so the cyanobacteria blooming present in this ecosystem can be considered of inoculation to these microorganisms for the waters downstream from the reservoir, wich will reflect problems to water treatment stations and the public health, if present cyanobacterial strains producing cianotoxins. Before of these facts become essential the constant monitorament of cyanobacteria and cyanotoxins at this ecosystem.
Conteúdo e variação sazonal de carboidratos ocorrentes na flora da Mata Atlântica do Espírito Santo
(Universidade Federal do Espírito Santo, 2006-02-22) Clippel, Joscineia Kelli; Cuzzuol, Geraldo Rogério Faustini; Zaidan, Lílian Beatriz Penteado; Thomaz, Luciana Dias
In Plants, the main storage carbohydrates include starch, soluble sugar, fructans and cell wall polysaccarides. Moreover, carbohydrates act like energetic substrate and structural compounds, many of these sugars can act as a protection against environmental stress in growth periods. Carbohydrates are used in food, pharmaceutical, cosmetic and textile industries. Although they have wide economic and ecologic importance, relatively little is known about their yield in plants native to the Atlantic present, especially in Espírito Santo (ES). This study was performed on order to contribute to more information. Non-structural carbohydrates were analysed from underground organs of the herbaceous Dioscorea sp. 1 (Dioscoriaceae), Dioscorea sp. 2 (Dioscoriaceae), Hedychium coronarium J. Koening (Zingiberaceae), Hippeastrum reticulatum Herb. (Amaryllidaceae), Prescottia nivalis Barb. Rodr. (Orquidaceae), Scadoxus multiflorus Martyn (Amaryllidaceae) and Sinningia aghensis Chautems (Gesneriaceae). To evaluate the cell wall polysaccarides, seeds of the herb Canavalia rosea L. and of the tree species Cassia fistula L., Cassia grandis L., Erythrina variegata L., Hymenaea coubaril L. and Ormosia arborea (Vell.) Harms (Leguminosae) were used. The soluble carbohydrates seasonal variation was performed in S. aghensis tuberculum. On the whole, Dioscoriaceae tuberous plants are rich in starch, reaching 50% in Discorea sp. 1 dry mass (DM). The species that exhibited the greatest amounts of fructose were the bulbous H. reticulatum (25% DM) and S. multiflorus (8,5% DM) and the rhizomatous P. nivalis (6,7% MS). The biggest soluble sugar purports, especially sucrose (63% DM), also were seen in P. nivalis. The tuberous Dioscorea sp. 2 and S. aghensis and the rhizomatous Hedychium coronarium presented the lowest total sugar and starch values, which could present another carbohydrates order on the analysed season. The high fructose content in H. reticulatum suggests the presence of fructans. From the cell wall storage polysaccaride purport analysed, the greatest purports were found in Hymenea coubaril seeds (70% DM), followed by Cassia grandis (50% DM) and Canavalia rosea (40% DM). E. variegata and O. arborea exhibited the lowest values of these compounds, representing 10 and 3% of DM respectively. Thus, H. coubaril, C. grandis and C. rosea appear take potential species in polysaccaride production for technological purposes. In carbohydrates seasonal variation analyses of S. aghensis tuberculum, the starch were 5,5% in autumn and 77,7% in winter, as of period which was reported lowest precipitation (3,6 mm). The total sugar soluble purport, sucrose and fructose were higher in summer, decreasing 50% in winter. As to glucose, greatest concentrations were found in autumn, followed by a conspicuous decrease in summer. The high concentration of starch in winter suggests that it may be linked to drought tolerance mechanisms.
Respostas ecofisiológicas da comunidade perifítica in situ a diferentes condições ambientais no Rio Santa Maria da Vitória, ES
(Universidade Federal do Espírito Santo, 2006-02-22) Martins, Fabíola Chrystian Oliveira; Fernandes, Valéria de Oliveira; Barroso, Gilberto Fonseca; Moschini-Carlos, Viviane
The physiological ecology responses of periphyton in the high course of the Santa Maria da Vitória (ES) river had been evaluated during one year, in two stations. The determined environmental variables had been: temperature, transparency, euphotic zone, depth, pH, OD, electric conductivity, STD, NO2 -, NO3 -, total-N, PO4 - and total-P. The Periphyton in natural substratum was evaluated through the biomass, chemical composition and structure of the algae community and classified through the autotrophic and Lakatos indexs. The river presented acid to slight acid waters, low electric conductivity and STD. OD, transparency and euphotic zone was raised. The total-N was high, had been compared with the ones of P, resulting in raised ratio NT/PT, evidencing an environment limited for P. The ACP showed distinction between the stations. It had difference of the periphytic biomass between the two stations explained, mainly, for the availability of nutrients. They had been registered 147 taxa of algae. Zygnemaphyceae was the Class most representative, qualitatively, in the station 1 and Cyanophyceae and Euglenophyceae in station 2. Bigger density was attributed to the Bacillariophyceae in the station 1 and Cyanophyceae in station 2. Zygnemaphyceae presented greater biovolume in the station 1 and Bacillariophyceae in station 2. During all the sampling period, in both the stations, the community was classified as heterotrophic and of low biomass. Station 1 presented trend to the trophic degree minor if compared station 2. This difference was the main factor that influenced the biomass and structure of the algae community, that if it showed limited, mainly, for P.
Respostas ecofisiológicas e bioquímicas de duas cultivares de tomate (Lycopersicum esculentum MILL.) cultivadas em sistemas de agricultura natural e convencional
(Universidade Federal do Espírito Santo, 2006-02-23) Tavares, Nelson Salgado; Ventura, José Aires; Costa, Helcio; Fernandes, Patrícia Machado Bueno
Today the world has interest in consuming healthful foods without chemical residues to maintain both the health of the human being and the planet Earth. The alterations that occur in tomato plants (Lycopersicum esculentum Mill.) cultivated in both natural and conventional systems of production had been assessed in two cultivars Gaúcho and Special Salada in Vila Velha, Espírito Santo, Southeast of the Brazil. The leaves and the fruits had been analyzed in the laboratories of official institutions, where was determined the weight, volume, humidity, dry matter, ashes, dry matter without ashes, total protein, free amino acids, total soluble solids, phenolic compounds, nitrate, the loss of water, time of shelf life and the macro and micronutrients. In the field, both photosynthesis and chlorophyll had been measured. It did not have production in the first plantation from natural farming. However, after four cultivations repeated in the same seedbeds, the fourth production was 21 t/ha. The first production of fruits of the conventional system was 56 t/ha and 167% greater than natural farming. The increase of the productivity in the natural farming was dependent of the capacity of the tomato plants adapt itself to the environment without chemical fertilizer and without poisons. The total phenol production within leaves and fruits of the tomato cultivated in system of natural farming was 36.86% greater than plants of tomato produced in the conventional system, while the total free amino acid was 305.53% greater in leaves and fruits growing in the conventional system. The plants of the natural farming had had greater water use efficiency (WUE) to fix CO2. The plants of the conventional system had used up 714 mL of water to fix 1.0 g CO2, while the plants of the natural farming had used up 321 mL to fix 1.0 g CO2. The plants of the system of natural farming had produced more vegetal matter for unit of natural resources than the plants of the system of conventional system. The fruits of the natural farming had had 16.76% more potassium, the biggest time of shelf life and had kept more characteristics of quality per 6 days than fruits of the conventional system.
Influência da modalidade de enxertia e interação entre enxertos e porta-enxertos em mudas de Theobroma cacao L. submetidas a um ciclo de défice hídrico
(Universidade Federal do Espírito Santo, 2006-03-07) Marinato, Claudio Sergio; Silva, Diolina Moura; Aguilar, Marco Antonio Galeas; Almeida, Alex Alan Furtado de
The cocoa tree (Theobroma cacao L.) is comercially cultivated in large scale (great quantities). Nowadays, a production breakdown, caused by a prague named witches’ broom, came up with new challenges to the farmers. An option of strategy used to face it is the substitution of vulneable plants for more resistant genotypes. A good way to proceed this process is the use fo grafting techniques to accelerate the substitution of the tree pantry or to the speed uo the production of the trees. This method is also used to obtain plants with the desirable characteristics, more tolerant to dry weather periods and greater productivety. However, the success of grafting can be influenced by many factors, including the interaction between the graft and the rootstock, which can be influenced by both genotypes. The main goal was evaluating the level of resistance of the T. cacao grafts, applying two methods of precocious grafting, and the physiological characteristics of grafted trees when submitted to few quantities of water. The experience was conducted in green houses with controlled temperature and relative humidity of air. There have been used vegetable material the genotypes ESFIP 02, TSH 1188, CCN 51 and ‘Common Cocoa’. There have been tests of hipocotiledonar grafting by wrip grafting and soft wood. Taxes of survival and characteristics of the vegetative growth were analised. The experience was installed in delineation of randomized blocks with four repetitions in factorial arrangement 2x5, based on two two grafting methodsand four rootstocksadded to a frank foot. The percentage of survival was not influenced by the grafting methods. In the precociuos grafting the rootstock genotype influenced, meaningfully, the survival of genotypes TSH 1188 grafts. The ESFIP 02 genotupes, the grafting method of soft wood provided greater growth than the wrip grafting method. On the other hand, the hidric deficit reduced the quantities of height, leaf area, the stem dry mass and the leaves dry mass. The dry mass from the roots was higher in the stressed trees. The stress decreased the levels of leaf nitrogen; the levels of phosphorus and potassium increased, though. The hidric deficit influenced the levels of chlorophill, its fkuorescence, the gas exchanges and the leaves hidric potential negativelly. O rootstock genotype influenced meaningfully the tolerance to hidric deficit and in the analised topics. The ESFIP 02 genotype, when used as rootstock permitted, generally higher levels of vegetative growth, dry mass accumultated, the leaves hidric potencial, chlorophill fluorescence and liquid photosynthesis
Estudo comparativo dos aspectos florísticos e fitossociológicos das restingas do Espírito Santo e da salinidade no crescimento inicial de Canavalia rosea (sw.) DC
(Universidade Federal do Espírito Santo, 2006-03-31) Zambom, Oberdan; Thomaz, Luciana Dias; Cuzzuol, Geraldo Faustini; Hebling, Selma Aparecida
The Praia de Caraís’ present in the Paulo César Vinha Stat Park was studied in the present work, in the district of Guarapari/ES, contemplating the halófila-psamófila vegetal zone in your floristic and fitossociologic aspects. A sampling area about 200 m² was evaluate through quadrate method (1m²) in 20 lines of ten square meters perpendiculars to the shore, from the vegetation begin to continent interior. In the Praia de Caraís were found 17 species in 11 botanic families. 32 species in 19 families were reveled in comparison with the Espírito Santo cost-line. The most representative families in numbers of species in Caraís and in the comparison done a long the Espírito Santo shoreline was: Poaceae, Fabaceae and Amaranthaceae and Convolvulaceae. From the species found as in past studies as in actual study, Remiria marítima, occurred in the 14 regions studied in the shoreline Espírito Santo. Others species like Panicum racemosum, Sporobolus virginicus, Remiria maritima, Canavalia rosea, Ipomoea imperati, Ipomoea pes-capre, it are considered widely shared in the Espírito Santo shoreline restinga, whereas Cassita filifrmis, Hidrocotile bonariensis, Chrysobalanus icaco had restricted distribution, and Hidrocotile umbelata, Alagoptera arenaria, Scaevola plumieri, Cereus fernambucensis wold be of low distribution. From the species sampled through the Espírito Santo cost-line, Allagoptera arenaria, Scaevola plumieri, Cassita filiformis e Chrysobalanus icaco had sampling distribution restrict to the Paulo César Vinha reserve biology, whereas others species like Canavalia rosea, Ipomoea imperati, Ipomoea pes-capre, Blutaparom portulacoides, Stenotaphrum secundatum, Panicum racemosum had wide distribution in the restingas sampled in Espírito Santo state. Canavalia rosea is the specie with the highest importance worth (IV 36,39) recorded to the actual Praia de Caraís segment. In the others places compared of the Espírito Santo cost this specie kept positions oscillating between the forth and the eighth place in IV. Ipomoea imperati, Panicum racemosum, Stenotaphrum secundatum and Remiria maritima were others species sampled in Caraís, with the highest IV, Relative Frequency and Relative Dominance. These species were found between the six most important in the halófilas-psamófilas restingas from the Espírito Santo state. As to salinity tested in Canavalia rosea, we observed that the salinity increase and/ or the electric conductivity inhibited the plant growth, decreasing the stem, root, leaves dry mass, the total dry mass, the length, the root: shoot ratio and the area leaf. The best performance was obtained in 0 mM and 200 mM of NaCl, occasioning serious damages, including the died of this plants in 28 and 56 days. About the 200 mM treatment, the plants survived to salinity, however with restrictions in the dry mass and others parameters evaluated. The growth rates (RGR and NAR) were affected with the soil salinity increase, showing decrease a long the experiment time. Restrictions also were found to specific area leaf salt, specific leaf mass (SLM) and area leaf rates (ALR), being more affected in the highest salinities. Thus, the ALR and SAL increased with the saliniry in face of dry matter lost, occurring the inverse with SLA, which decreased. As to chlorophyll and carotenoids purport, were not seen variations in concentration after 20 days in 0 mM and 200 mM treatments. Suggesting the others photosynthetic and photoactive mechanisms were affected compromising the specie growth. Hence we can verify that the salinity is one of the factors that promoted unbalances in Canavalia rosea plants.
Propriedade antifúngica de extrato protéico de folhas do abacaxizeiro
(Universidade Federal do Espírito Santo, 2006-06-12) Pereira, Umberto Zottich; Fernandes, Patricia Machado Bueno; Ventura, José Aires; Tardin, Flávio Dessaune; Batitucci, Maria do Carmo Pimentel
Although Ananas comosus (L.) Merr. is a fruit tree that has a great economic importance in Brazil it faces some limitations mainly in agriculture illness especially those that are caused by fungi. Thus, a fusariosis resistant genotype (EC-099) was selected by INCAPER. Nevertheless resistant factor of this plant is not clear yet. So, extract proteins from chlorophylls tissues of the plant leafs and test their activities against fungi of economic interest. Protein extraction was made followed by the separation by molecular weight trough precipitation with ammonium sulfate resulting in fractions 0-20% (F1), 20- 50% (F2) and 50-75% (F3) p/v. The antifungal activity was evaluated during 4 days through in spectrophotometer analysis by determination of fungi growth Aspergilus niger, Beauveria bassiana, Colletotrichum gloesporioides, Colletotrichum musae, Chalara paradoxa, Cladosporium sp., Fusarium subglutinanas f. sp. ananas (E-261) resistant and proneness to the fungicidal Benomyl, Penicilium sp. e Trichophyton rubrum grown in a potato dextrose (BD) liquid medium, inoculated with 1,5x106 spores/ml. Concentrations of 0; 0,05; 0,1; 0,2; 0,3; 0,4 and 0,5 mg/ml of each fraction were tested. F1 inhibited growth of all tested fungi that were, obtaining the minor media and differing statistically from the other fractions when tested against A. niger, C. gloesporioides, C. musae, Cladosporium sp., F. subglutinanas f. sp. ananas, Penicilium sp. and T. rubrum. The development of C. paradoxa was ~ 30% inhibited by the three fractions, and had no significant difference among them. The F2 and F3 fractions presented a higher specificity to A. niger and Cladosporium sp. However, F2 and F3 were not efficient to inhibit the growth of F. subglutinanas f. sp. ananas ( less them 20%). F3 inhibited the A. niger growth up to 42,9%. By the regression analysis a better inhibition’s linearity by F1 was observed, obtaining higher values of the coefficient of correlation linear, when compared with F2 and F3, excepted to fungi B. bassiana e C. paradoxa. The results obtained in the tests demonstrated the existence of proteins with antifungal action in the resistant genotype of the pineapple tree (EC-099) and F1 presented higher efficiency in fungal inhibition. Thus, we may deduce that these proteins might be associated with the resistance of this genotype to fusariosis. Furthermore, the results demonstrate the existence of proteins with a high specter of action against different lends of fungi, indicating that this is an important object of research in genetic and in pharmacological industry.

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