Ciências Veterinárias

URI Permanente desta comunidade

http://repositorio.ufes.br/handle/10/17470
Programa de Pós-Graduação em Ciências Veterinárias

Centro: CCAE
Telefone: (28) 3552 8983
URL do programa: http://www.cienciasveterinarias.ufes.br/pos-graduacao/PPGCV

Navegar

Navegando Ciências Veterinárias por Assunto "Acetato de medroxiprogesterona"

Agora exibindo 1 - 2 de 2
  • Nenhuma Miniatura disponível
    Item
    Atividade secretória e histologia uterina de cadelas pré-púberes com ablação parcial do desenvolvimento das glândulas endometriais
    (Universidade Federal do Espírito Santo, 2013-08-26) Martins, Bianca Barcelos; Luz, Marcelo Rezende; Neves, Mariana Machado; Siqueira, Jeanne Broch
    The use of steroids with actions in the female reproductive system may affect the development and differentiation of the uterus, which can lead to abnormal development of endometrial glands, with decreased reproductive efficiency and even sterility. Thus, this methodology could be applied in the population control of dogs. However, in cases of partial inhibition of the development of the glands is not known effects of steroids on the functionality formed glands, as well as the possible occurrence of endometrial fibrosis. This study aimed to evaluate the secretory activity of the endometrial glands of prepubertal bitches undergoing a protocol for partial ablation of endometrial glands development. We used sixteen female mongrel bitches from different litters, distributed in the MPA group (n = 8), females that received injections of medroxyprogesterone acetate (10mg/kg/SC), every 3 weeks, from day 1 after birth until the age of six months, and C (n = 8), control females, untreated, which received applications of 0.9% NaCl solution. At 6 months of age, ovariohysterectomy was performed in all animals, and samples of uterine horns were collected and fixed for histology and histochemistry. To evaluate the intensity of uterine secretion (grades 1-4), the stains of periodic acid Schiff (PAS) and Alcian Blue (AB) pH 2.5 were used. For histological analysis we used Masson s trichrome and toluidine blue staining. Statistical analysis was performed by Kruskal-Wallis test, using the R software, with significance level of 5%. No dog in both groups exhibited cystic endometrial hyperplasia (HCE) and presence of content in the uterine lumen. For PAS only the grades 1 (absent) and 2 (mild) were observed in both groups, without difference between groups. However, the MPA showed greater intensity of uterine secretion (grade 2) in comparison to the C group (p <0.05). With respect to AB pH 2.5, in both groups we also found only the grades 1 (absent) and 2 (mild), without statistical difference between groups. There was no Masson s trichrome staining in the periglandular region in both groups. There was greater amount of mast cells present in the region of the myometrium in relation to the endometrium in both groups. It is concluded that prepubertal bitches with partial ablation of uterine adenogenesis present minimal uterine secretory activity, absence of uterine periglandular fibrosis and presence of larger amount of mast cells in the myometrium in relation to the endometrium
  • Nenhuma Miniatura disponível
    Item
    Efeito do acetato de medroxiprogesterona sobre o desenvolvimento das glândulas endometriais em cadelas: avaliações histológica e imunoistoquímica.
    (Universidade Federal do Espírito Santo, 2013-02-01) Teixeira, Natália Soares; Luz, Marcelo Rezende; Siqueira, Jeanne Broch; Neves, Mariana Machado
    Bitches with uterus devoid of endometrial glands, phenotype called uterine glandknockout (UGKO), should be sterile animals, and could contribute to the species overpopulation control. Since the inadequate exposure of the female reproductive system to steroids during critical periods of development can lead to the formation of this phenotype, as observed in cows, sheep and rats, the aim of this study was to evaluate the effect of serial applications (from birth to 6 months old) of medroxyprogesterone acetate on the development of endometrial glands in the bitch. Sixteen cross-bred females, from different litters, were distributed in the MPA group (n = 8), females that received injections of medroxyprogesterone acetate (10mg/kg/SC), every 3 weeks, from day 1 after birth to age of 6 months, and C (n = 8), control females, untreated, that received injections of 0.9% NaCl solution, treated similarly. At age of 6 months, ovariohysterectomy was performed in all animals, and samples of uterine horns were collected and fixed for histologic and immunohistochemical analyzes. We measured the total thickness (µm) from the uterine wall, endometrium (µm); myometrium (µm), diameter of the endometrial glands (µm), number of endometrial glands / mm2, epithelial height (µm) and expression of ER –a, ER-ß and PR in the surface epithelium and endometrial stroma. Bitches from MPA group showed 35% reduction in the number of endometrial glands, endometrial glands with larger diameter, greater uterine wall, endometrium and myometrium thickness, and the height of the epithelium. There were no statistical differences between groups in relation to the expression of ER-a, ER-ß and PR in both the surface epithelium and in endometrial stroma. It is concluded that serial applications of medroxyprogesterone acetate, from birth to 6 months old are not able to cause total ablation in the development of endometrial glands in the bitch, but a 35% reduction in its development.