Mestrado em Bioquímica

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http://repositorio.ufes.br/handle/10/17562

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Agora exibindo 1 - 20 de 34

A deficiência de testosterona atenua os efeitos do remodelamento cardíaco tardio após o infarto agudo do miocárdio em ratos
(Universidade Federal do Espírito Santo, 2018-05-28) Fernandes, Rafaela de Araújo; Bittencourt, Athelson Stefanon; Soares, Aurélia Araújo Fernandes; Batista, Priscila Rossi; Guimaraes, Marco Cesar Cunegundes
abstract
Alterações comportamentais, bioquímicas e moleculares em modelo animal de inalação crônica de "crack" : papel dos sistemas dopaminérgico e endocanabinóide no córtex pré-frontal
(Universidade Federal do Espírito Santo, 2014-07-17) Azevedo, Lorena Bianchine Areal de; Martins e Silva, Cristina; Pires, Rita Gomes Wanderley; Bittencourt, Ana Paula Santana de Vasconcellos; Ribeiro, Fabiola Mara
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Análise da expressão de potenciais inflamassomas envolvidos na quimiorresistência adquirida à cisplatina em modelo in vitro de carcinoma de ovário seroso de alto grau
(Universidade Federal do Espírito Santo, 2018-10-08) Silva, Ana Maria Rodrigues da; Rangel, Letícia Batista Azevedo; Salgado, Breno Souza; Lyra Junior, Paulo Cilas Morais
High-grade serous ovarian cancer (HGS-OC) is the most frequent cause of deaths among gynecological malignancies. Due to their asymptomatic development as well as the lack of sensitive and specific screening methods, the disease is diagnosed in advanced and incurable stages. Although tumors usually respond to first line taxanes and platinum-based chemotherapy, most patients develop relapse and chemoresistance. Inflammation has been involved in the initiation and development of many types of cancer, including HGS-OC. Recently, the protein components of the innate immune system, known as inflammassome, have been associated with the mechanisms of progression and metastasis of neoplasias, however, it’s role in drugresistance is little explored. In this study, through RT-PCR and western blot analysis we investigated the clinical significance of inflammassomas in modulating the chemoresistant phenotype in cisplatin-resistant cells (ACRP) obtained from the parental line of HGS-OC (A2780). Our results have shown that, even though mRNA expression levels of NLRP3 remained unaltered in the ACRP, the NLRP3 protein expression appears to vary in a dose-dependent way with cisplatin treatment, although it does not configure the main event in chemoresistance. An increase in mRNA expression levels of the NLRP1, but not of NLRC4, in resistant cell line ACRP was also obtained. In conclusion, our study was the first to show the involvement of NLRP1 inflammassome in the development and regulation of cisplatin-resistance in HGS-OC cells, making it an attractive target with great therapeutic potential against the disease.
Avaliação da expressão dos genes DNAHC6, DYNLT1, Pcdh21, SIGMAR1, Lingo1 e Gabra2 como marcadores moleculares na Doença de Huntington
(Universidade Federal do Espírito Santo, 2018-09-27) Mendonça, Suellen Rosseto; Pires, Rita Gomes Wanderley; Bem, Daniela Amorim Melgaço Guimarães do; Silva, Cristina Martins e; Muniz, Marcelo Ramos; Errera, Flavia Imbroisi Valle
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Avaliação dos efeitos neuroquímicos e comportamentais do enriquecimento ambiental em animais submetidos a privação maternal
(Universidade Federal do Espírito Santo, 2017-07-14) Lima, Randriely Merscher Sobreira de; Bittencourt, Athelson Stefanon; Bittencourt, Ana Paula Santana de Vasconcellos; Santos, Jeyce Willig Quintino dos; Harres, Vanessa Beijamini; Bortoli, Valquíria Camin de
The relationship between mother and its pups is extremely important for mammals, given the importance of maternal care and attachment in the first days of life. Traumatic events during this period may impair physiological and psychological development, potentially causing short- and long-term changes. Maternal deprivation (MD) is a well-established protocol used to investigate both neurobiological and behavioral changes such as anxiety disorders. It has been demonstrated that environmental enrichment (EE) protocols promote numerous sensory, motor, and cognitive benefits in laboratory animals, and may be used to intervene in changes caused by postnatal adverse events and to prevent the occurrence of psychiatric disorders in adulthood. In this context, we evaluated the implications of EE as a strategy to prevent the maternal deprivation effects on anxiety behaviors and gene expression of the serotonergic system components.
Avaliação dos efeitos tóxicos da exposição ao inseticida clorpirifós sobre as respostas cardiovasculares e comportamentais em animais experimentais
(Universidade Federal do Espírito Santo, 2014-07-23) Cunha, Alexandre Frinhani; Sampaio, Karla Nívea; Andrade, Tadeu Uggere de; Moraes, Leonardo Resstel Barbosa
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Avaliação dos parâmetros comportamentais e de estresse oxidativo em animais submetidos à separação maternal e ao consumo de etanol em binge durante a adolescência
(Universidade Federal do Espírito Santo, 2016-09-16) Telles, Vanessa Ghidetti Alvarenga; Rodrigues, Lívia Carla de Melo; Bittencourt, Ana Paula Santana de Vasconcellos; Dalmaz, Carla; Gonçalves, Juliana Barbosa Coitinho
Early life stress can generate long-lasting effects on brain and behavior during adulthood. Furthermore, stressful experiences stimulate abusive consumption of ethanol, which induces neuronal damages and contributes to increases on parameters of oxidative stress on Central Nervous System. The aim of this research was to evaluate the effects of Maternal Separation (MS) and of intermittent ethanol binge drinking in adolescent rats on behavioral and oxidative stress parameters. Pregnant Wistar rats were used. After birth of litters, the animals were divided into control group (NS) or maternal separation group (MS). Maternal separation was performed from 2 to 15 postnatal day, 3 hours daily. At postnatal day 35, animals were divided again into 3 groups: animals which received intragastric saline (vehicle group) or ethanol in doses of 3.0 or 6.0 g/kg. Ethanol was administered once a day, in a regimen of two consecutive days interspersed by two days without ethanol, totalizing 10 doses for chronic binge treatments. Acute binge exposure happened at postnatal day 35 until 37 postnatal day.
Avaliação físico-química de nanopartículas de ouro reduzidas com citrato de sódio e capeadas com Virola oleifera
(Universidade Federal do Espírito Santo, 2017-06-30) Corrêa, Andressa dos Santos; Oliveira, Jairo Pinto de; Guimarães, Marco Cesar Cunegundes; Nogueira, Breno Valentim; Silva, André Romero da
The capping of gold nanocrystals using sodium citrate with Virola oleifera was the focus of this thesis, which aim/aiming at synthesize and characterize evaluate the final extract concentration and pH influence in surface particle adsorption. Furthermore, was analysed antioxidant activity and citotoxicity of AuNPs, for investigate your potential biological application. For this goal, the best capping time, and subsequently the optimum pH range and final extract concentration was determined and was realizes a 32 factorial design with 3 levels and 2 variables (pH and extract concentration) to verify the optimal capping conditions. In this way, it has been proved the adsorption was most intense between pH 3 and 5 and the most stable was the lower final extract concentration. The x-ray diffraction and the transmission electron microscopy confirmed the face-centered cubic structure of citrate AuNPs and the quasi spherical aspect, respectively. The adsorption of aromatic molecules was confirmed by spectroscopy techniques: Fourier-Transform Infrared (FTIR) and Raman. The DPPH and FRAP analyzes shows enhancement of antioxidant activity with capping of citrate gold nanoparticles. Furthermore, the citotoxicity assay shows low citotoxicity of capped gold nanoparticles.
Bases moleculares envolvidas na neuroproteção causada pela exposição a um ambiente enriquecido em modelo animal de isquemia cerebral
(Universidade Federal do Espírito Santo, 2017-07-07) Gonçalves, Lara Vezula; Pires, Rita Gomes Wanderley; Silva, Cristina Martins e; Massensini, Andre Ricardo; Nogueira, Breno Valentim
abstract
Caracterização físico-química da isoforma γ-tripsina bovina
(Universidade Federal do Espírito Santo, 2014-06-26) Lacerda, Caroline Dutra; Santos, Alexandre Martins Costa; Santana, Marcos Aurélio de Santana; Ribeiro, Joselito Nardy; Pires, Rita Gomes Wanderley
A novel bovine trypsin isoform was purified from commercial sample by ion exchange chromatography by Sephadex SP C50®. New isoform contain in addition of loss of N- terminus hexapeptide (as found in parent molecule β-trypsin) an intra-chain split between Lys-155 and Ser-156. The novel enzyme denominate gamma-trypsin showed similar properties with α-trypsin isoform in: polypeptide number chain (two chain), molecular masses (23,312 Da), secondary structure, hydrodynamic radius and others. In spite of enzymatic and structural similarities of both isoforms, -trypsin preferably has a lower rate formation from β-trypsin, a lower surface charge, but the gamma-trypsin has a higher thermal stability than α-trypsin. Due to obtaining facility of purification of bovine trypsin isoforms from commercial font, and properties described above, become this enzyme an interesting alternative for the food industry, detergent and biocatalysis research.
Caracterizações bioquímica e hemostática de pacientes com diabetes Mellitus Tipo 2 em insulinização
(Universidade Federal do Espírito Santo, 2014-07-15) Gonçalves, Nadmy Arrivabene Zavaris; Bem, Daniela Amorim Melgaço Guimarães do; Vassalo, Breno Valentim; Gomes, Isabele Beserra Santos
The study aims to characterize biochemically and hemostatic mellitos patients with diabetes mellitus type 2 (DM2) who developed insulin regimen. Composed group under analysis, 40 patients attended the Basic Health Unit of Consolation, Vitória / ES, aged 25 to 80 years, diagnosed with DM2 and that were already evoluted to insulinization. As controls, 40 patients were selected in the same age group without laboratory and / or clinical diagnosis of DM. Markers of inflammation, hypercoagulability and fibrinolysis were measured: Fibrinogen, D-dimer (D-Di) and plasminogen activator type 1 inhibitor (PAI-1). The polymorphism (-675 4G/5G) in the promoter region of the PAI-1 gene was correlated with their serum levels. Biochemical parameters were measured: plasma glucose (PG), glycated hemoglobin (A1C), total cholesterol (TC), HDL cholesterol (HDL-C), LDL (LDLc) cholesterol, triglycerides (TGC), ultrasensitive C reactive protein (hsCRP), urea and serum creatinine. There was still checking BMI, obesity, smoking, hypothyroidism, hypertension, dyslipidemia and insulin resistance. Statistical analysis showed significant differences (p <0.05) between groups with regard to mean values of HDLC, VLDL-C, TGC, urea, hsCRP and fibrinogen. There was significant difference between groups for VLDLc, TGC, creatinine and fibrinogen. Controls present correlations between: fibrinogen and glucose, hsCRP and fibrinogen, PAI-1 and glucose, PAI-1 and BMI. In insulinization DM2 group correlation was observed correlation between: fibrinogen and D-di, hsCRP and fibrinogen, D-Di and glucose (negative), PAI-1 and triglycerides, PAI-1 and BMI. PAI-1 levels were higher in the control group in subjects with genotype 5G5G, 4G5G and 4G4G followed. Binary Logistic Regression confirmed that the variables hypertension and fibrinogen were significant at p-value (0.009) and (0.049) and adjusted odds ratio (4.184, 1.426 to 12.276) and (3.293, 1.006 to 10.775), respectively, showing that hypertensive patients have a risk 4.18 times more likely to have insulinization type 2 diabetes and that individuals with hyperfibrinogenemia have a 3.29 times greater risk. With this study we hope to contribute to better understanding of the complex changes that accompany the user insulinization type 2 diabetic patients in expectation of seeking appropriate treatment and prevention for the macrovascular complications of diabetes.
Coloração de espécimes anatômicos para aplicação no processo de plastinação por meio de corantes histológicos : floxina b, safranina, fucsina fenicada e tricrômico de masson
(Universidade Federal do Espírito Santo, 2018-09-17) Siqueira, Bruno Magela de Melo; Bittencourt, Ana Paula Santana de Vasconcellos; Bittencourt, Athelson Stefanon; Silva, André Romero da; Ribeiro, Joselito Nardy
The Study of Anatomy has been known for several centuries and before this, every moment has generated several repercussions in this area of biomedical sciences. Even with advancement in the technological sectors, the teaching of anatomical concepts through corpses in health courses is still essential. A more common way of preserving anatomical parts for studies is through a substance that was discovered in 1867 by German Hoffman by accident: formaldehyde, but such substance has an unpleasant odor and irritates the nasal passages and the eye region. Plastination is the newest in terms of technology for the preservation of anatomical specimens. This technique was developed by the German Dr. von Hagens in 1977 and its principles reflect in the idea of impregnating polymers (silicone, epoxy or polyester) in biological tissues, removing the fat and water present, thus increasing their durability and with aspects close to the original object. Plastination is free from toxic conservative substances, thus facilitating its role for didactic and scientific purposes. The dye is used for a better visualization of the structures in the anatomical part and thus ending with that aspect of worn, emphasizing the true color of the object. In view of the above, has developed a staining protocol in skeletal muscle tissue, applied to the Plastination technique with the following dyes: Phloxine B, Safranin, Phenicated Fuchsin and Masson's Trichrome. The interaction of the dye with the muscle tissue, fat and epithelial tissue of the samples that were used for the research was evaluated. All the dyes used in the macroscopic staining were able to show certain structures such as tendon, fascia and connective tissue inside the muscle. It was also evaluated the photodegradation of these dyes in solution. It was noticed that the dyes that obtained more satisfactory results in relation to the dyeing of the specimens were those of anionic character: Phloxine B and Masson's Trichrome, but in relation to the resistance of the dyes in light exposure, it was not possible to choose the one that obtained the best because the solutions exhibited different behaviors when exposed to light. The final protocol for the staining muscle tissues will be applied in the collection of the Life Sciences Museum of the Federal University of Espírito Santo and in the Department of Morphology of the same University.
Determinação da atividade e da estabilidade termodinâmica da isoforma α-tripsina bovina em meios aquo-orgânicos
(Universidade Federal do Espírito Santo, 2015-02-24) Pereira, Evaldo Vitor; Gonçalves, Juliana Barbosa Coitinho; Santos, Alexandre Martins Costa; Pires, Rita Gomes Wanderley; Fernandes, Patrícia Machado Bueno; Bemquerer, Marcelo Porto
abstract
Determinação de parâmetros estruturais e termodinâmicos da isoforma a-tripsina bovina em solventes aquo-orgânicos
(Universidade Federal do Espírito Santo, 2017-02-23) Rosa, Dayanne Pinho; Gonçalves, Juliana Barbosa Coitinho; Santos, Alexandre Martins Costa; Fernandes, Patrícia Machado Bueno; Cicilini, Maria Aparecida; Bem, Daniela Amorim Melgaço Guimaraes do
Organic solvents are common in industrial processes that use enzymes but, at the same time, it is known that they change the properties thereof, thus the effects of aqueous-organic solvent (ethanol) in different concentration on the α-trypsin structure have been investigated by spectroscopic techniques and thermodynamic data analysis. The results from spectroscopic measurements, including far-UV Circular Dichroism, UV-Vis absorption spectroscopy, intrinsic tryptophan fluorescence and dynamic light scattering (DLS) suggest the formation of partially folded states, instead of aggregates states, at high ethanol concentration (> 60% v/v ethanol), with little loss of secondary structure, but with significant tertiary structure changes. The thermodynamic data (Tm and ∆H) suggest a loosen of intramolecular weak interactions, which reflects in a flexibility increase such that the catalytic capacity can be increased or decreased according to the ethanol concentration into the system. Overall results we suggest that in range of 0-60% v/v ethanol/buffer, α-trypsin undergoes reversible multimerization phenomena maintaining its catalytic activity. However from 60% v/v ethanol/buffer, population of folded partially states with less catalytic activity are predominant.
Efeito da privação de sono paradoxal e do bloqueio da síntese da corticosterona sobre os limiares da reação de defesa induzida pela estimulação elétrica da matéria cinzenta periaquedutal e colículo superior
(Universidade Federal do Espírito Santo, 2015-08-19) Barbosa, Rafaella Vila Real; Bittencourt, Ana Paula Santana de Vasconcellos; Bittencourt, Athelson Stefanon; Harres, Vanessa Beijamini; Santos, Jeyce Willig Quintino dos
Panic Disorder (PD) is a common mental disorder that affects up to 5% of the population at some point in life and is characterized by the presence of recurrent panic attacks (AP). It is a psychopathology that may be affected by sleep deprivation (PS), relation that is still poorly understood. In this context, experimental models of AP and PS are useful tools in investigating this possible correlation, especially motivated by the growing of deprivation of sleep, which has become increasingly common in modern society. This study evaluated the effects of paradoxical sleep deprivation (PSP) in the thresholds of defensive behaviors induced by intracranial stimulation (EI) of MCPD and CS in rats, which is an experimental model of AP, as well as verified the influence of corticosterone on these thresholds. 160 male Wistar rats were used (300g), organized into 4 groups of 40 animals each, as follows: control group (CTR) submitted to EI, but no PSP; Deprivation group (PRV), submitted to EI and sleep deprived for 96 hours; Deprivation Group + corticosterone synthesis inhibition (PRB), undergoing treatment with metyrapone, EI, and sleep deprived for 96 hours, and the control group + corticosterone synthesis inhibition (CTB), undergoing treatment with metyrapone and EI, but without sleep deprivation. After 10 days of intracranial surgical implant of electrode in MCPD, the animals underwent 5 stimulation sessions, as follows: 1st (TRI) considered as a screening session - immediately before the deprivation, 2nd (P48) after 48 hours of deprivation, 3rd (P96) after 96 hours of deprivation, 4th (R48) 48 hours after the end of deprivation, and 5th (R96) after 96 hours of withdrawal of deprivation. The thresholds of the individual curves obtained for defense responses in the various stimulation sessions of CS and MCPD (TRI, P48, P96, R48 and R96) of the rats were compared, as well as a given threshold response curves in different groups (CTR, PRV, CTB and PRB). Furthermore, the levels of corticosterone (CORT) were measured in different sessions of EI and compared in the same group, as well as between the different groups. In the CTR group, all behaviors were equal in all sessions when compared to the TRI, however, in deprived animals (PRV), the threshold for galloping (GLP) was significantly reduced in R48 and R96, without changes in other behaviors. In contrast, in the xxi PRB group, Trotting (TRT) increased from P48, while the GLP has not changed in any EI session. Comparing the groups, Jumping (SLT), Micturation (MIC), Exophthalmos (EXO), Immobility (IMO), Defecation (DEF), Trotting (TRT) and Galloping (GLP), were not altered in function of CORT levels produced due PSP, suggesting that corticosterone does not influence the characteristic defensive behaviors induced by electrical stimulation of MCPD and CS. In addition, these results suggest that the delayed effect of the PSP on the GLP thresholds is possibly due to time-dependent neurochemical mechanisms.
Efeito da separação maternal sobre a impulsividade, consumo voluntário de etanol e expressão de componentes do sistema endocanabinóide e dopaminérgico em córtex pré-frontal e hipocampo : influência do consumo de etanol em binge na adolescência
(Universidade Federal do Espírito Santo, 2016-09-16) Mata, Martielo Januário da; Bittencourt, Ana Paula Santana de Vasconcellos; Dalmaz, Carla; Pires, Rita Gomes Wanderley
Maternal separation is an animal model used to mimic stressful events in the neonatal period, which may lead to the development of cognitive impairments and substances abuse, such as ethanol. This substance is a potential risk to health due to its high consumption by young people, and its consumption alters behavior and neurotransmitter systems function, such as dopaminergic and endocannabinoid systems. In this study, we evaluate the influence of ethanol binge drinking in adolescent rats subjected to maternal separation on learning, impulsivity and voluntary ethanol consumption in adulthood, as well as (the effects of these treatments) on the endocannabinoid and dopaminergic systems. For this, male Wistar rats were separated from their mothers or not (control group) during the postnatal days (DPN) 2-15, for 3 hours daily. Animals not separated were kept on animal facility reared conditions. At DPN 35, the animals were divided in acute or chronic treatment. Both groups were again divided into 3 subgroups, which receive vehicle (saline) or ethanol in doses of 3.0 or 6.0 g/kg by intragastric administration. Ethanol was administered during three consecutive days (acute treatment) or once a day, two consecutive days, interspersed by two days without ethanol, totalizing 10 doses (chronic treatment). At the end of this procedure, the animals were subjected to behavioral tests (learning and impulsivity tests), both using a T-maze, and to the voluntary ethanol consumption test, or euthanized for prefrontal cortex and hippocampus removal. The mRNA expression of the components of the endocannabinoid system: CB1, monoacylglycerol lipase, fatty acid amide hydrolase, N-acylphosphatidylethanolamine phospholipase D and diacylglycerol lipase were evaluated in both structures, and the dopaminergic receptors D1, D2 and tyrosine hydroxylase enzyme were evaluated only in the prefrontal cortex. We observed that maternal separation increased impulsivity behavior and voluntary consumption of ethanol, and ethanol in adolescence impaired short-term memory and appears to reverse other behavioral changes due to maternal separation. In the prefrontal cortex, maternal separation and ethanol altered dopaminergic system with reduction of D1 mRNA expression and increased tyrosine hydroxylase, and appears to increase the enzymes for endocannabinoid synthesis, N-acyl-phosphatidylethanolamine phospholipase D e diacylglycerol 17 lipase. In the hippocampus, the group submitted to both treatments presented a reduction of CB1 mRNA expression and the enzyme N-acylphosphatidylethanolamine phospholipase D, and an increased expression of diacylglycerol lipase. In conclusion, maternal separation and ethanol were able to cause behavioral changes and modifications in endocannabinoid and dopaminergic systems, and maternal separation modifies ethanol effects response.
Efeito de diferentes agonistas de receptores galaninérgicos no núcleo dorsal da rafe de ratos expostos a modelos animais de ansiedade e pânico
(Universidade Federal do Espírito Santo, 2015-02-26) Morais, Juliana da Silva; Harres, Vanessa Beijamini; Bittencourt, Athelson Stefanon; Andreatini, Roberto
Galanin (GAL) is a peptide present in the CNS of various mammals, including human being. Three different receptors have been cloned for the GAL, GALR1 and GALR3, inhibitory, and GALR2, excitatory. The distribution of GAL in structures involved in the control of emotions, and behavior studies suggest that GAL may be involved in the neurobiology of anxiety. The effect seems to depend on both the GAL administration site as involved receptor subtype. The dorsal raphe nucleus (DRN) is distinguished by the presence of serotonergic neurons, important to mediate the antidepressant effect of several drugs. About 40% of the neurons of the DRN co-expressing serotonin and GAL. The activation of GALR1 in this structure decreases the firing rate of serotonergic neurons in the DRN. Previous results from our laboratory showed anxiolytic effect of GAL intra-DRN in rats exposed to the elevated T maze (ETM), but no effect on the escape, related to panic. One of the limitations of this model is that the escape (latency to leave the open arm) usually occurs within a few seconds, so that detect panicogenic effect (low latency escape) can be difficult. Thus, one aim of this study was to investigate the effect of intra-DRN rat GAL in another experimental model of panic, electrical stimulation of the dorsal periaqueductal gray matter (DPAG), more sensitive to detect panicogenic effect. Furthermore, given the existence of different subtypes of galaninergic receptors (GALR1 and GALR2) in the DRN with opposite transduction mechanisms, inhibitory and excitatory, respectively, it is possible that activation of GALR1 receptors is responsible for mediating the anxiolytic/panicogenic effect while activation of GALR2 receptors induces anxiogenic/panicolytic effect. Accordingly, we tested this hypothesis using a selective agonist for GALR1 (M617) and a selective agonist for GALR2 (AR-M1896) in the DRN in rats exposed to ETM. The administration of M617 1.0 and 3.0 nmol in the DRN facilitated inhibitory avoidance, suggesting an anxiogenic-like effect., while administration of AR-M1896 3,0nmoles in the DRN impaired the inhibitory avoidance, suggesting an anxiolytic-like effect, both without changing locomotor activity of animals tested in the open field. Also there was no effect of these drugs on the ETM escape behavior. Later, the pre-treatment with WAY100635 was tested in order to verify that administration of a 5-HT1A antagonist would be able to block the effects seen with GALR2 agonist in animals exposed to ETM. The anxiolytic effect of AR-M1896 was attenuated by the prior administration of WAY100635 at a dose of 0,18nmol. Therefore these results suggest a relationship between the observed effect with the AR-M1896, the release of 5-HT and activation of 5-HT1A receptors. And finally, the GAL 0,3nmol intra-DRN increased significantly the jumpping and trotting thresholds of animals submitted to electrical stimulation of the DPAG, suggesting a panicolytic effect. Together, the results show that the GAL in the DRN participates in the mediation of behavioral responses related to anxiety and, less clearly, to Panic Disorder. The results also shows that effect of GAL on anxiety depends galaninergic subtype receptor activated.
Efeito de diferentes tempos de exposição a um ambiente enriquecido na memória de camundongos : uma abordagem comportamental e molecular
(Universidade Federal do Espírito Santo, 2015-12-11) Rohor, Bruna Zanetti; Martins e Silva, Cristina; Pires, Rita Gomes Wanderley; Bittencourt, Ana Paula Santana de Vasconcellos; Rodrigues, Lívia Carla de Melo
The environment is formed by a set of situations around the individual and has influence on all organisms. A stressor environment has negative influences in a subject, but an enriched environment (EE) has positive influences. In animals, the AE is defined as a combination of inanimate and social complex stimuli that can improve motor, cognitive and sensory functions. The EE is composed of different objects of different shapes, sizes, colors, textures, among others. Environmental novelty, voluntary physical activity and social interaction are also part of EE. The EE is capable of promoting increased visual, sensory, cognitive and motor functions. Cognitive improvement, increased synaptic plasticity, adult hippocampal neurogenesis, modulation of gene expression and changes in levels of neurotransmitters and neurotrophic factors are EE-induced alterations already described. However, several EE paradigms are found in the literature, and this variety of protocols produces a range of controversial results on the effects induced by EE.
Efeitos comportamentais da cetamina em ratos expostos ao labirinto em T elevado : possível envolvimento da via de sinalização de BDNF/TrkB na matéria cinzenta periaquedutal (MCP)
(Universidade Federal do Espírito Santo, 2016-04-06) Silote, Gabriela Pandini; Joca, Sâmia Regiane Lourenço; Harres, Vanessa Beijamini; Hott, Sara Cristina; Schenberg, Luiz Carlos
abstract
Efeitos da exposição a um ambiente enriquecido sobre parâmetros comportamentais, bioquímicos e moleculares em um modelo murino da doença de Parkinson
(Universidade Federal do Espírito Santo, 2015-08-05) Hilário, Willyan Franco; Silva, Cristina Martins e; Pires, Rita Gomes Wanderley; Nakamura-Palacios, Ester Miyuki; Rodrigues, Lívia Carla de Melo
abstract

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