Mestrado em Ciência e Tecnologia de Alimentos
URI Permanente para esta coleção
Nível: Mestrado Acadêmico
Ano de início: 2011
Conceito atual na CAPES: 4
Ato normativo: Homologado pelo Parecer CES/CNE nº 487/2018 (Portaria MEC nº 609, de 14/03/2019) e Publicado DOU em 18/03/2019 (Seção 1, pág. 63).
Periodicidade de seleção: Semestral
Área(s) de concentração: Ciência e Tecnologia de Alimentos
Url do curso: https://cienciaetecnologiadealimentos.ufes.br/pt-br/pos-graduacao/PPGCTA/detalhes-do-curso?id=1076
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Navegando Mestrado em Ciência e Tecnologia de Alimentos por Assunto "Açaí"
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- ItemAvaliação do efeito da sonicação em enzimas na polpa de açaí (Euterpe edulis)(Universidade Federal do Espírito Santo, 2016-02-17) Furtado, Maraysa Rodrigues; Silva, Pollyanna Ibrahim; Carvalho, Raquel Vieira de; São José, Jackline Freitas Brillante deThe non-thermal treatments are promising techniques for the processing of food. Ultrasound is a able to minimize changes during processing and storage of food. The application of ultrasonic waves in foods can inactivate enzymes and microorganisms, as well as assist in the extraction of components. Acai is a fruit with high nutritional value and commercial demand, but easily loses some of its original properties due enzymatic reactions. This study aimed to evaluate and compare the sonication techniques, thermosonication and conventional heat treatment for inactivation of peroxidase and polyphenol oxidase and maintenance of physical-chemical properties of acai pulp. Were evaluated, Moisture content, pH, titratable acidity, lipids anthocyanins, phenolic compounds and reducing sugars activity of peroxidase and polyphenol in acai pulp treated and not treated with ultrasound and heat treatment. For thermosonication temperatures used were 20, 30, 40, 50, 60 ° C for 10 minutes. It was observed that the thermosonication and heat treatment were able to maintain the characteristics of color, pH, water content, lipid, titratable acidity and anthocyanins. Application of treatment with ultrasound at 20 ° C in acai pulp was obtained behavior similar to the heat treatment in the peroxidase activity. While the nectar, none of the treatments showed statistical significance when compared to the control sample. Treatment with ultrasound is promising only if the parameters that interfere with the efficiency of propagation of sound waves, such as soluble solids content and concentration of the components are reduced.
- ItemAvaliação do potencial anti-inflamatório e antioxidante da casca da jabuticaba (Myrciaria cauliflora), do açaí jussara (Euterpe edulis Martius) e do jambolão (Syzygium cumini) em camundongos submetidos à dieta de cafeteria(Universidade Federal do Espírito Santo, 2014-07-25) Sant'Ana, Marcella Ramos; Silva, Pollyanna Ibrahim; Costa, Neuza Maria Brunoro; Costa, André Gustavo Vasconcelos; Cintra, Dennys Esper CorreaIncreased body fat is associated with increased inflammation and oxidative stress, which may compromise the health of the individual. An alternative to try to restore the oxidative and inflammatory balance of the body would be the intake of antioxidant compounds such as polyphenols present in fruits such as jaboticaba (Myrciaria cauliflora), the jambolan (Syzygium cumini) and jussara açai (Euterpe edulis Martius), fruit with low consumption, but with a large production in southeastern Brazil. The objective of this study was to investigate the effect of supplementation of these fruits on oxidative stress and inflammation in animals fed a high calorie and high fat diet. The fruits were lyophilized and subsequently evaluated its chemical composition and concentration of total phenolics, anthocyanin and antioxidant activity. For the biological assay, 30 adult male mice of the Swiss race divided into 5 groups (n = 6/group) were used, namely: group treated with standard commercial diet (negative control), group treated with cafeteria diet (positive control) and test groups that received the cafeteria diet plus 2% freeze-dried jaboticaba peel powder or jambolan or acai jussara for 14 weeks. After the experimental period was evaluated serum antioxidant capacity and lipid peroxidation as well as the levels of the anti-inflammatory (IL-10) and proinflammatory cytokines (TNF-a and IL-6). It was applied t test to compare the results of the control groups and ANOVA, complemented with Tukey test (a = 5%), to compare the results between the tests groups and the positive control. The freeze-dried acai jussara powder was the fruit with higher concentrations of anthocyanins, while the jaboticaba peel showed the highest concentrations of phenolic compounds and antioxidant activity in vitro. Supplementing with freeze-dried fruits was effective in increasing serum antioxidant activity in experimental animals, but no reduction of serum lipid peroxidation was observed. Regarding the inflammatory profile, no difference between the animals supplemented with freeze-dried fruits and receiving only cafeteria diet was observed. It can be concluded that the fruits tested were effective in increasing serum antioxidant activity of the supplemented animals, but showed no anti-inflammatory effect in these animals.
- ItemEfeito da jabuticaba (Myrciaria caulifora), do fruto da palmeira juçara (Euterpe edulis Martius) e do jambolão (Syzugium cumini) sobre o perfil lipídico, a glicemia e a endotoxemia em camundongos submetidos à dieta de cafeteria(Universidade Federal do Espírito Santo, 2015-02-24) Constancio, Vanessa da Silva; Costa, Neuza Maria Brunoro; Viana, Mirelle Lomar; Costa, André Gustavo Vasconcelos; Lomar, Viana, MirelleThe excess body fat leads to an inflammatory reaction associated with metabolic endotoxemia and increased insulin resistance, as well as altering the lipid profile which results in damages to liver and renal function. Studies suggest that intake of antioxidant foods, such as polyphenols, provide beneficial effects on glucose and lipid metabolism. The objective of this study was to investigate the jabuticaba bark effect (Myrciaria cauliflora), pulp of açai juçara (Euterpe edulis Martius) and jambolan (Syzygium cumini) on the lipid profile, blood glucose and endotoxemia in Swiss mice underwent diet cafeteria. Initially, the fruits were freeze-dried and evaluated for their chemical composition. The biological assay included 50 adult male mice of the Swiss race divided into 5 groups (n = 10 / group), namely: the group treated with standard commercial diet (negative control), group treated with cafeteria diet (positive control) and groups test for 14 weeks who received the cafeteria diet supplemented with 2% jabuticaba bark, or pulp jambolan or pulp açai juçara lyophilized. In the 13th and 14th week were determined tolerance to insulin and glucose of animals. At the end of the experiment, we assessed weight gain, blood biochemistry, histopathology and endotoxemia. The biochemical parameters were: total cholesterol (TC) and HDL-C fractions, LDL-C, triglycerides (TAG) and C-reactive protein (CRP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Histopathology studies the effects of calorie diet over the area of adipocytes, fatty liver and kidney function from the number and area of the glomeruli. The endotoxemia was assessed by the concentration of lipopolysaccharide (LPS) in serum of the animals. We used the t test for comparison of results between the control group and ANOVA, and Tukey's test (a = 5%), to compare the groups supplemented with fruit and the positive control. Supplementation with 2% jambolan the cafeteria diet resulted in a significant reduction (p <0.05) CT content, LDL-C, TAG, the TC / HDL and decreased adipocyte animal area treated with the fruits. The supplementation of açai juçara was also able to reduce the content of TC, IGT, and adipocytes area, in addition to increasing glucose tolerance. On the other hand, the blemish was not effective in improving the lipid parameters related to metabolism, glucose metabolism and histopathological aspects. Supplementation with 2% lyophilized fruits did not cause positive effects in reducing weight gain, insulin resistance and endotoxemia caused by ingestion of a cafeteria diet. Furthermore, the fruits were not effective in preserving renal histology and fatty infiltration of the liver. It follows that the inclusion of jambolan and açai juçara in the diet can have positive effects on the damage caused by high fat diets, especially with regard to dyslipidemia, glucose tolerance and adipocyte hypertrophy.
- ItemEfeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica(Universidade Federal do Espírito Santo, 2016-07-11) Silva, Laíz Aparecida Azevedo; Costa, Neuza Maria Brunoro; Oliveira, Daniela da Silva; Costa, André Gustavo Vasconcelos; Nunes, Erika Takagi; Hermsdorff, Helen Hermana MirandaCardiovascular diseases (CVD) are the major responsible factor for morbidity and mortality rates worldwide. The intake of foods rich in antioxidants, such as acai, can help reducing the risk of CVD. The objective of this study was to investigate the effect of the jussara acai (Euterpe edulis Martius) on cardiovascular risk biomarcador in mice submitted to a high fat diet. The pulp of jussara acai was lyophilized and analysed for its chemical composition, phenolic compounds, anthocyanins and antioxidant capacity. For the biological assay, 43 adult male Swiss mice were used. Initially, the animals were submitted a control diet (AIN-93M) (n = 22) or high fat diet (n = 21) for 8 weeks. For a period of additional 8 weeks, the group received the control diet (AIN-93M): control diet (AIN-93M) (CT, n = 10) or control diet (AIN-93M) supplemented with 2% lyophilized jussara açai pulp (CT+A, n = 12); the group received high fat diet: high fat diet (HF, n = 9) or high fat diet supplemented with 2% lyophilized jussara acai pulp (HF+A, n = 12). At the end of the experiment the animals were euthanized and blood samples collected for analysis: aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol, HDL-C, LDL-C, triglycerides, free fatty acids, glucose, activator inhibitor plasminogen type 1 (PAI-1), intercellular cell adhesion molecule (ICAM), e-selectin, were histologically analyzed liver and adipose tissue. The results were analyzed by t test to evaluate the control and HF groups prior to supplementation. The 'two-way' analysis of variance (ANOVA) was performed to evaluate the effect of diet and fruit and/or its interaction among the experimental groups (CT, HF, CT+A and HF+A). The data were presented as mean and standard error, with 5% significance level. The experimental groups CT, CT+A and HF+A had less than 33% of hepatocytes with lipid accumulation, however, the intensity of hepatic steatosis HF group was classified between 33 and 66% of the hepatic parenchyma with fat deposition. As for the area of adipocytes was no effect of diet, fruit or the interaction between the experimental groups. Supplementation with 2% lyophilized jussara açaí was not effective to improve the levels of AST, ALT, total cholesterol, HDL-C, LDL-C, triglycerides, free fatty acids and glucose. The jussara açaí did not change cardiovascular risk biomarcador ICAM, PAI-1 and e-selectin. In conclusion, supplementation with acai jussara was not effective in improving cardiovascular risk biomarcador and other biochemical parameters evaluated. On the other hand, acai jussara promoted reduction of hepatic steatosis of the mice subjected to high-fat diet.
- ItemIogurte simbiótico de açaí (Euterpe edulis Mart.) : caracterização físico-química e viabilidade de bactérias láticas e probiótica(Universidade Federal do Espírito Santo, 2015-04-08) Leite, Sabrina Torres; Bosi, Mirela Guedes; Fantuzzi, Elisabete; Roberto, Consuelo Domenici; Bernardes, Patricia Campos; Queiroz, Vagner Tebaldi de; Pires, Ana Clarissa dos SantosThe symbiotic yogurt, which combine probiotic microorganisms and prebiotic substances, added with fruit pulp are a growing trend in the market. The acai fruit (Euterpe edulis) stands out by the presence of bioactive compounds, such as anthocyanins. In this context, this study aimed to characterize and evaluate the physical and chemical parameters and microbiological feasibility of the symbiotic acai yogurt enriched with inulin and added with probiotic culture of Bifidobacterium animallis subsp. lactis BB-12. The yogurt formulations met the physicochemical requirements of the IN n° 46/2007 by MAPA, presenting ash content of 0,86% ± 0,10, total dry extract 23,18% ± 2,59, fat content 4,16 ± 0,31%, 0,70% ± 0,05 of acidity and 4,45 ± 0,10 pH. Among the formulations, the total phenolic content ranged from 18,17 to 117,84 mg AGE / 100g, anthocyanins from 1,92 to 47,88 mg /100 g and antioxidant activity from 0,71 to 6,95 µmol Trolox / g, observing an increase according to the increase of the added acai pulp content. After 28 days of storage at 5° C, there was a decrease in anthocyanin content and antioxidant activity. There was a positive contribution of acai pulp in viability of the lactic bacteria total whose scores ranged from 4,56 to 7,04 log CFU.g-1) and B. lactis BB-12 ranged from 3,17 to 6,34 log CFU.g-1, favoring the multiplication of these bacteria in the yogurts. In the formulations, added with 20 and 25% of acai pulp, the counting of the lactic bacteria total and probiotic bacteria, remained viable according to the IN n° 46/2007 by MAPA and the Functional Property Claim List (Anvisa), during 28 days of storage at 5 °C. It was concluded that the addition of acai pulp E. edulis, inulin and B. lactis BB-12 it was technologically feasible in the elaboration of symbiotic acai yogurt, and a great alternative to diversify the product on the market.