Mestrado em Agroquímica
URI Permanente para esta coleção
Nível: Mestrado Acadêmico
Ano de início: 2016
Conceito atual na CAPES: 4
Ato normativo: Homologação da 161ª Reunião do CTC-ES, Parecer CNE/CES 617/2016.1502
Periodicidade de seleção: Anual
Área(s) de concentração:Agroquímica
Url do curso: https://agroquimica.alegre.ufes.br/pt-br/pos-graduacao/PPGAQ/detalhes-do-curso?id=1056
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- ItemBiocarvão de exocarpo de sapucaia (lecythis pisonis) para a adsorção de verde brilhante(Universidade Federal do Espírito Santo, 2024-02-23) Campos, Phelipe Augusto Carvalho; Costa, Anna Isabel Guido; https://orcid.org/0000-0003-4745-9719; Profeti, Luciene Paula Roberto; https://orcid.org/0000-0001-6280-2410; Camêlo, Danilo de Lima; Gardiman Júnior, Benvindo SirtoliIn contemporary times, the escalating processes of industrialization and urbanization have led to watercourses' contamination by persistent organic pollutants (POPs), such as dyes, pharmaceuticals, pesticides, and aromatic hydrocarbons. Consequently, water quality is significantly compromised. To mitigate these adverse impacts, adsorption emerges as an effective and economically viable technique for treating effluents contaminated with POPs. The choice of adsorbent material is pivotal for the development of an adsorption-based decontamination process. In this context, biocarbons possess suitable characteristics as adsorbents, including high surface area, mechanical stability, and surface functional groups that act as binding sites for various organic species. With the aim of contributing to the development of sustainable technologies, this study primarily focused on the synthesis and performance evaluation of a biocarbon produced from the exocarp of Sapucaia (Lecythis pisonis) for removing the dye Brilliant Green (VB). The biocarbon synthesis, utilizing Sapucaia exocarp as a precursor, occurred at a temperature of 600°C under a nitrogen atmosphere. The synthesized material was characterized through thermogravimetric analysis (TG), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Surface area (BET and BJH) and pH at the point of zero charge (pH_PZC) were also determined. Adsorption kinetic tests for VB were conducted at temperatures of 25, 35, 45, and 55°C, using a dye solution at a concentration of 250 ppm and a pH of 7. Characterization results indicated that the synthesized material exhibited a considerable surface area and the presence of crucial surface functional groups, fundamental for successful adsorption processes. The adsorption tests revealed that the synthesized biocarbon exhibited high efficacy in removing the dye from aqueous solutions. Among the nonlinear kinetic models applied to the experimentally obtained data, the Avrami model more accurately described the VB adsorption process. According to this model, the adsorption kinetics are fractional order. Moreover, the analysis of parameters related to the Weber and Morris diffusion model indicated that the dye adsorption kinetics are controlled by more than one mechanism, supporting the Avrami model. The Sips model proved to be the most suitable for fitting the experimental equilibrium results, and thermodynamic results suggested that the adsorption process is endothermic, spontaneous, and irreversible. This study underscores the potential of biocarbon derived from Sapucaia exocarp as a promising solution for the effective removal of organic pollutants, such as Brilliant Green, from aqueous solutions. Additionally, it emphasizes the need for future research, given the limited knowledge available in the literature on this material, offering opportunities for further studies and enhancements.
- ItemDesenvolvimento e efeito de emulsões contendo compostos bioativos sobre Fusarium oxysporum f. sp. lycopersici e qualidade fisiológica de sementes de tomate(Universidade Federal do Espírito Santo, 2024-01-23) Peccini, Luan Rezende; Costa, Adilson Vidal ; https://orcid.org/; http://lattes.cnpq.br/; Pires, Raquel Maria de Oliveira ; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador3; https://orcid.org/; http://lattes.cnpq.br/; Queiroz, Vagner Tebaldi ; https://orcid.org/; http://lattes.cnpq.br/; Orientador2; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Soares, Lucas de Souza ; https://orcid.org/; http://lattes.cnpq.br/; Flores, Rafaela Venancio ; https://orcid.org/; http://lattes.cnpq.br/; 3º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 4º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 5º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 6º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 7º membro da banca; https://orcid.org/; http://lattes.cnpq.br/Tomato is the solanaceous crop frequently affected by various pathogens, including the fungus Fusarium oxysporum f. sp. lycopersici (Fol), which causes Fusarium wilt and reduces productivity. In this study, different formulations containing thymol (Thy) or carvacrol (Car) were developed and their effects on Fol and the physiological quality of tomato seeds were evaluated. Thy emulsions (EsT) were developed considering surfactant concentration, vortex homogenization time (HVtx) and high-intensity ultrasound time (HHUs). EsT9%S1 emulsion, comprising 9.0% surfactant, 2.5 minutes of HVtx and 3.0 min of HHUs, kept kinetic stability over a 28 day storage period. EsT9%S1 showed antifungal activity against Fol at inhibitory concentrations of IC50 = 0.052 mg mL -1 and IC90 = 0.268 mg mL-1 . Seeds treated with EsT9%S1 maintained electrical conductivity (EC) and pH in the range of 240.9 to 597.7 μS cm-1 g -1 and 6.8 to 7.6, respectively over 24h. Additionally, no signs of phytotoxicity were observed in the tomato seeds. Coatings using cassava starch (CS) and glycerol (Glyc), incorporating different concentrations of carvacrol nanoemulsion (CNE) and/or red cabbage extract (RCE) were developed and characterized. Analysis of the FTIR and XRD spectra showed interactions among CS, Glyc, CNE, and/or RCE, indicated by shifts/emergence of new bands, and the appearance of amorphous peaks. The incorporation of CNE, and/or RCE made the film's surface more rough, increased thermal stability and EC, decreased pH, and modified solubility and swelling properties. Films with the addition of CNE and/or RCE exhibited antioxidant activity, an important property for protecting seeds against oxidative stress. Physiological analyses revealed the absence of phytotoxicity in the coatings applied to the tomato seeds. The results highlight the potential of EsT9%S1 and the films containing CS, Glyc, CNE, and/or RCE for evaluation as tomato seed coatings
- ItemEstrutura supramolecular entre 2-hidroxipropil-β-ciclodextrina e citral potencializa a atividade contra Rhipicephalus microplus (Acari: Ixodidae)(Universidade Federal do Espírito Santo, 2024-02-15) Bigui, Walter Cesar Celeri; Soares, Lucas de Souza ; https://orcid.org/; http://lattes.cnpq.br/; Costa, Adilson Vidal ; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador3; https://orcid.org/; http://lattes.cnpq.br/; Queiroz, Vagner Tebaldi de ; https://orcid.org/; http://lattes.cnpq.br/; Orientador2; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Almeida, Luiz Cláudio de ; https://orcid.org/; http://lattes.cnpq.br/; Flores, Rafaela Venancio ; https://orcid.org/; http://lattes.cnpq.br/; 3º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 4º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 5º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 6º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 7º membro da banca; https://orcid.org/; http://lattes.cnpq.br/Rhipicephalus microplus is responsible for significant damage to global livestock, and the control of this parasite faces barriers such as acaricide resistance. Citral (CIT) is a potential acaricide; however, due to characteristics such as low volatility and water dispersibility, its field application is limited. The objective of this study was to prepare, characterize, and evaluate, in vitro, the acaricidal activity of the complex containing citral and 2-hydroxypropyl-β-cyclodextrin on R. microplus larvae. The complex (CIHβCIT) by kneading and the physical mixture (MFHβCIT) were prepared in a stoichiometric ratio of 1:1, confirmed by the solubility diagram and the Job Plot method. The intermolecular interaction between CIT and HPβCD was indicated, suggesting complex formation, confirmed by FTIR-ATR, TGA/DTG, DSC, XRD, and SEM. The kneading preparation method produced CIHβCIT with high yield (90.60%), encapsulation efficiency (72.03%), and drug loading of 6.48%. These parameters were determined through UV-Vis spectroscopy. The LC50 values obtained by the larval packet test for CIT, CIHβCIT, and MFHβCIT were 0.66%, 1.13%, and 1.16% (w/v), respectively. The formation of CIHβCIT reduced the volatility of CIT, maintaining acaricidal activity (81.59%) over 48 hours, compared to free CIT (64.61%) and MFHβCIT (60.86%). Thus, the preparation of CIHβCIT through the kneading technique is capable of enhancing and ensuring the acaricidal activity of CIT for an extended period
- ItemEficiência de biofiltros na qualidade da água em aquicultura(Universidade Federal do Espírito Santo, 2024-03-04) Marques, Viviane dos Santos; Co-orientador1; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador2; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador3; https://orcid.org/; http://lattes.cnpq.br/; Orientador1; https://orcid.org/; http://lattes.cnpq.br/; Orientador2; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; 1º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 2º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 3º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 4º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 5º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 6º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 7º membro da banca; https://orcid.org/; http://lattes.cnpq.br/Aquaculture has been experiencing accelerated growth in recent years due to the demand for sustainable food sources and preservation of natural resources. Advances in technologies and management practices have improved efficiency and productivity in aquaculture, contributing economically and environmentally. No water recirculation system (RAS) is continuously recycled and treated to maintain ideal quality, through mechanical and biological filters, in addition, it allows the control of environmental parameters, optimizing various aspects of cultivation. Therefore, the objective of this work is to analyze the influence of different biological filter substrates on maintaining water quality and performance of juvenile Nile tilapia cultivated in RAS. To this end, the study was divided into three chapters, which address the zootechnical performance of Nile tilapia, the physical-chemical quality of the water and the use of eggshell as an alternative substrate for biofilters, respectively. The results obtained were subjected to analysis of variance and a significant effect was selected. The means were compared using the Tukey tests at 5% and Duncan tests at 10% significance. The use of eggshell as a substrate was efficient and stood out among other treatments, providing its economic and environmental predictions when used as a filtering medium, requiring further research into its handling and durability. For CE and SST, values above those recommended by legislation were recorded. For ammonia, critical analyzes were observed up to 30 days, after this period the biofilters reached complete maturation and were efficient in converting ammonia into nitrite and nitrate, maintaining the production levels of these substances at the end of the experiment. It is concluded that all treatments provide satisfactory conditions for performance and water quality, especially those composed of different proportions of clay and gravel, reflecting on the well-being and growth of the animals
- ItemEstudos por QSAR, docagem e dinâmica molecular de derivados de quinazolina tendo como alvo a proteína beta tubulina para o controle da fusariose(Universidade Federal do Espírito Santo, 2024-02-16) Gasques, Larissa de Souza; Co-orientador1; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador2; https://orcid.org/; http://lattes.cnpq.br/; Co-orientador3; https://orcid.org/; http://lattes.cnpq.br/; Orientador1; https://orcid.org/; http://lattes.cnpq.br/; Orientador2; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; 1º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 2º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 3º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 4º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 5º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 6º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 7º membro da banca; https://orcid.org/; http://lattes.cnpq.br/Gibberellosis or ear fusariosis is a disease caused by phytopathogenic fungi such as Fusarium graminearum. Significant losses in agriculture and impacts on the global economy are evidenced by the disease, which is minimized by the use of commercial fungicides. In this scenario, it is important to create new fungicides that are more efficient and, preferably, less harmful to the environment. This work describes computational studies applied to a set of quinazoline-derived molecules that were synthesized and previously evaluated for their fungicidal activity against F. graminearum in the literature. The selected compounds served as prototypes for the construction of the 2D and 3D QSAR models (CoMFA and CoMSIA), where satisfactory results were obtained during validation. Topological descriptors (JGI1 and VE3_D) were able to predict the QSAR-2D model with q² = 0.743, r² = 0.797 and r²test = 0.764. In CoMFA, q² = 0.834, r² = 0.958, and r²test = 0.834 were obtained with the set of molecules loaded by the RESP HF 6-311G method. The best model in CoMSIA, on the other hand, was derived from atomic partial charges using the Gasteiger-Marsili empirical method, obtaining a q² = 0.956, r² = 0.987 and r²test = 0.858 using the electrostatic and hydrophobic descriptors. The QSAR models were used to predict the biological activity of triazole compounds from the library belonging to our research group. The derivatives that stood out at this stage were sent for molecular docking analysis, which revealed the intermolecular interactions at the compounds' binding sites and compared them with the fungicide carbendazim. This study revealed that compound T15 was favorable to toxicity tests by ProTox II, as well as by the QEPest program. Then, a 200 ns molecular dynamics simulation was carried out to evaluate the behavior and stability of compound T15 and to compare it with carbendazim. The analyses suggest that the triazole (a thymol derivative) was stable during the simulation, performing more intermolecular interactions at the β-tubulin active site than the commercial fungicide. MM/GBSA results predict that its binding free energy was -44 kcal/mol, while carbendazim -18 kcal/mol. The results reveal that T15 proved to have an ability to inhibit fusarium, since its interaction with the protein is stronger. Therefore, it can be concluded that the models built in this work were able to predict and identify a promising compound with optimal predicted biological activity